Solution structure of the dimeric cytoplasmic domain of syndecan-4

J. Shin, W. Lee, D. Lee, B. K. Koo, I. Han, Y. Lim, A. Woods, J. R. Couchman, E. S. Oh

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Abstract

The syndecans, transmembrane proteoglycans which are involved in the organization of cytoskeleton and/or actin microfilaments, have important roles as cell surface receptors during cell-cell and/or cell-matrix interactions. Since previous studies indicate that the function of the syndecan-4 cytoplasmic domain is dependent on its oligomeric status, the conformation of the syndecan-4 cytoplasmic domain itself is important in the understanding of its biological roles. Gel filtration results show that the syndecan-4 cytoplasmic domain (4L) itself forms a dimer stabilized by ionic interactions between peptides at physiological pH. Commensurately, the NMR structures demonstrate that syndecan-4L is a compact intertwined dimer with a symmetric clamp shape in the central variable V region with a root-mean-square deviation between backbone atom coordinates of 0.95 Å for residues Leu186-Ala195. The molecular surface of the 4L dimer is highly positively charged. In addition, no intersubunit NOEs in membrane proximal amino acid resides (C1 region) have been observed, demonstrating that the C1 region is mostly unstructured in the syndecan-4L dimer. Interestingly, two parallel strands of 4L form a cavity in the center of the dimeric twist similar to our previously reported 4V structure. The overall topology of the central variable region within the 4L structure is very similar to that of 4V complexed with the phosphatidylinositol 4,5-bisphosphate; however, the intersubunit interaction mode is affected by the presence of C1 and C2 regions. Therefore, we propose that although the 4V region in the full cytoplasmic domain has a tendency for strong peptide-peptide interaction, it may not be enough to overcome the repulsion of the C1 regions of syndecan-4L.

Original languageEnglish
Pages (from-to)8471-8478
Number of pages8
JournalBiochemistry
Volume40
Issue number29
DOIs
Publication statusPublished - 2001 Jul 24

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All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Shin, J., Lee, W., Lee, D., Koo, B. K., Han, I., Lim, Y., Woods, A., Couchman, J. R., & Oh, E. S. (2001). Solution structure of the dimeric cytoplasmic domain of syndecan-4. Biochemistry, 40(29), 8471-8478. https://doi.org/10.1021/bi002750r