Solution structure of the tetrameric minimum transforming domain of p53

Weon Tae Lee, Timothy S. Harvey, Ya Yin, Patrick Yau, David Litchfield, Cheryl H. Arrowsmith

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

We report the solution structure of the minimum transforming domain (residues 303-366) of human p53 (p53tet) determined by multidimensional NMR spectroscopy. This domain contains a number of important functions associated with p53 activity including transformation, oligomerization, nuclear localization and a phosphorylation site for p34/cdc2 kinase. p53tet forms a symmetric dimer of dimers that is significantly different from a recent structure reported for a shorter construct of this domain. Phosphorylation of Ser 315 has only minor structural consequences, as this region of the protein is unstructured. Modelling based on the p53tet structure suggests possible modes of interaction between adjacent domains in full-length p53 as well as modes of interaction with DNA.

Original languageEnglish
Pages (from-to)877-890
Number of pages14
JournalNature Structural Biology
Volume1
Issue number12
DOIs
Publication statusPublished - 1994 Jan 1

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Phosphorylation
Dimers
Intrinsically Disordered Proteins
Oligomerization
Nuclear magnetic resonance spectroscopy
Phosphotransferases
Magnetic Resonance Spectroscopy
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Genetics

Cite this

Lee, W. T., Harvey, T. S., Yin, Y., Yau, P., Litchfield, D., & Arrowsmith, C. H. (1994). Solution structure of the tetrameric minimum transforming domain of p53. Nature Structural Biology, 1(12), 877-890. https://doi.org/10.1038/nsb1294-877
Lee, Weon Tae ; Harvey, Timothy S. ; Yin, Ya ; Yau, Patrick ; Litchfield, David ; Arrowsmith, Cheryl H. / Solution structure of the tetrameric minimum transforming domain of p53. In: Nature Structural Biology. 1994 ; Vol. 1, No. 12. pp. 877-890.
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Lee, WT, Harvey, TS, Yin, Y, Yau, P, Litchfield, D & Arrowsmith, CH 1994, 'Solution structure of the tetrameric minimum transforming domain of p53', Nature Structural Biology, vol. 1, no. 12, pp. 877-890. https://doi.org/10.1038/nsb1294-877

Solution structure of the tetrameric minimum transforming domain of p53. / Lee, Weon Tae; Harvey, Timothy S.; Yin, Ya; Yau, Patrick; Litchfield, David; Arrowsmith, Cheryl H.

In: Nature Structural Biology, Vol. 1, No. 12, 01.01.1994, p. 877-890.

Research output: Contribution to journalArticle

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