Solution structures of the N-terminal domain of histone H4

E. Bang, C. H. Lee, J. B. Yoon, D. W. Lee, Weontae Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Histones, nuclear proteins that interact with DNA to form nucleosomes, are essential for both the regulation of transcription and the packaging of DNA within chromosomes. The N-terminal domain of histone H4 contains four acetylation sites at lysine residues and may play a separate role in chromatin structure from the remainder of the H4 chain. We performed circular dichroism and NMR characterization of both native (H4NTP) and acetylated (Ace-H4NTP) peptides containing N-terminal acetylation domain of histone H4 for various pH environments. Data from CD and NMR suggested that H4NTP exhibited a pH-dependent conformational change, whereas the Ace-H4NTP is insensitive to pH change. However, both peptides showed a defined structural form at acidic pH environments. The solution structure for Ace-H4NTP shows two structurally independent regions comprising residues of Leu10-Gly13 and Arg19-Leu22, demonstrating relatively well-defined turn-type structures. Our results suggest that N-terminal acetylated region of H4 prefers an extended backbone conformation at neutral pH, however, upon acetylation, the regions containing lysine residues induce structural transition, having defined structural form for its optimum function.

Original languageEnglish
Pages (from-to)389-398
Number of pages10
JournalJournal of Peptide Research
Volume58
Issue number5
DOIs
Publication statusPublished - 2001 Dec 10

Fingerprint

Acetylation
Histones
Lysine
Nuclear magnetic resonance
Peptides
Nucleosomes
DNA
Transcription
Chromosomes
Nuclear Proteins
Chromatin
Conformations
DNA Packaging
Packaging
Circular Dichroism

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology

Cite this

Bang, E. ; Lee, C. H. ; Yoon, J. B. ; Lee, D. W. ; Lee, Weontae. / Solution structures of the N-terminal domain of histone H4. In: Journal of Peptide Research. 2001 ; Vol. 58, No. 5. pp. 389-398.
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Solution structures of the N-terminal domain of histone H4. / Bang, E.; Lee, C. H.; Yoon, J. B.; Lee, D. W.; Lee, Weontae.

In: Journal of Peptide Research, Vol. 58, No. 5, 10.12.2001, p. 389-398.

Research output: Contribution to journalArticle

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