Sonophoresis using ultrasound contrast agents: Dependence on concentration

Donghee Park, Gillsoo Song, Yongjun Jo, Jongho Won, Taeyoon Son, Ohrum Cha, Jinho Kim, Byungjo Jung, Hyunjin Park, Chul Woo Kim, Jongbum Seo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Sonophoresis can increase skin permeability to various drugs in transdermal drug delivery. Cavitation is recognized as the predominant mechanism of sonophoresis. Recently, a new logical approach to enhance the efficiency of transdermal drug delivery was tried. It is to utilize the engineered microbubble and its resonant frequency for increase of cavitation activity. Actively-induced cavitation with low-intensity ultrasound (less than ∼1 MPa) causes disordering of the lipid bilayers and the formation of aqueous channels by stable cavitation which indicates a continuous oscillation of bubbles. Furthermore, the mutual interactions of microbubble determined by concentration of added bubble are also thought to be an important factor for activity of stable cavitation, even in different characteristics of drug. In the present study, we addressed the dependence of ultrasound contrast agent concentration using two types of drug on the efficiency of transdermal drug delivery. Two types of experiment were designed to quantitatively evaluate the efficiency of transdermal drug delivery according to ultrasound contrast agent concentration. First, an experiment of optical clearing using a tissue optical clearing agent was designed to assess the efficiency of sonophoresis with ultrasound contrast agents. Second, a Franz diffusion cell with ferulic acid was used to quantitatively determine the amount of drug delivered to the skin sample by sonophoresis with ultrasound contrast agents. The maximum enhancement ratio of sonophoresis with a concentration of 1:1,000 was approximately 3.1 times greater than that in the ultrasound group without ultrasound contrast agent and approximately 7.5 times greater than that in the control group. These results support our hypothesis that sonophoresis becomes more effective in transdermal drug delivery due to the presence of engineered bubbles, and that the efficiency of transdermal drug delivery using sonophoresis with microbubbles depends on the concentration of microbubbles in case stable cavitation is predominant.

Original languageEnglish
Article numbere0157707
JournalPLoS One
Volume11
Issue number6
DOIs
Publication statusPublished - 2016 Jun 1

Fingerprint

Contrast Media
Drug delivery
Cavitation
Ultrasonics
drugs
microbubbles
Microbubbles
Pharmaceutical Preparations
ferulic acid
bubbles
Skin
Lipid bilayers
Bubbles (in fluids)
lipid bilayers
Natural frequencies
Lipid Bilayers
Experiments
Tissue
oscillation
Permeability

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Park, Donghee ; Song, Gillsoo ; Jo, Yongjun ; Won, Jongho ; Son, Taeyoon ; Cha, Ohrum ; Kim, Jinho ; Jung, Byungjo ; Park, Hyunjin ; Kim, Chul Woo ; Seo, Jongbum. / Sonophoresis using ultrasound contrast agents : Dependence on concentration. In: PLoS One. 2016 ; Vol. 11, No. 6.
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Park, D, Song, G, Jo, Y, Won, J, Son, T, Cha, O, Kim, J, Jung, B, Park, H, Kim, CW & Seo, J 2016, 'Sonophoresis using ultrasound contrast agents: Dependence on concentration', PLoS One, vol. 11, no. 6, e0157707. https://doi.org/10.1371/journal.pone.0157707

Sonophoresis using ultrasound contrast agents : Dependence on concentration. / Park, Donghee; Song, Gillsoo; Jo, Yongjun; Won, Jongho; Son, Taeyoon; Cha, Ohrum; Kim, Jinho; Jung, Byungjo; Park, Hyunjin; Kim, Chul Woo; Seo, Jongbum.

In: PLoS One, Vol. 11, No. 6, e0157707, 01.06.2016.

Research output: Contribution to journalArticle

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