Sox2 maintains epithelial cell proliferation in the successional dental lamina

Eun Jung Kim, Seo Yoon Jung, Zhaoming Wu, Sushan Zhang, Han Sung Jung

Research output: Contribution to journalArticle

Abstract

Objectives: The successional dental lamina is the distinctive structure on the lingual side of the vertebrate tooth germ. The aim of this study was to investigate the relationship among Sox2, Claudin10 and laminin5 and the role of Sox2 in successional dental lamina proliferation during vertebrate tooth development. Materials and Methods: To understand the successional dental lamina, two types of successional tooth formation, that in geckos (with multiple rounds of tooth generation) and that in mice (with only one round of tooth generation), were analysed. Results: Unique coexpression patterns of Sox2 and Claudin10 expression were compared in the successional dental lamina from the cap stage to the late bell stage in the mouse tooth germ and in juvenile gecko teeth to support continuous tooth replacement. Furthermore, Laminin5 expression was shown in the cap stage and decreased after the bell stage. Upon comparing the epithelial cell cycles and cell proliferation in successional dental lamina regions between mouse and gecko molars using BrdU and IdU staining and pulse-chase methods, distinctive patterns of continuous expression were revealed. Moreover, Sox2 overexpression with a lentiviral system resulted in hyperplastic dental epithelium in mouse molars. Conclusions: Our findings indicate that the regulation of Sox2 in dental lamina proliferation is fundamental to the successional dental lamina in both species.

Original languageEnglish
Article numbere12729
JournalCell Proliferation
DOIs
Publication statusAccepted/In press - 2019 Jan 1

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Tooth
Epithelial Cells
Cell Proliferation
Lizards
Tooth Germ
Vertebrates
Bromodeoxyuridine
Tongue
Cell Cycle
Epithelium
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Kim, Eun Jung ; Jung, Seo Yoon ; Wu, Zhaoming ; Zhang, Sushan ; Jung, Han Sung. / Sox2 maintains epithelial cell proliferation in the successional dental lamina. In: Cell Proliferation. 2019.
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Sox2 maintains epithelial cell proliferation in the successional dental lamina. / Kim, Eun Jung; Jung, Seo Yoon; Wu, Zhaoming; Zhang, Sushan; Jung, Han Sung.

In: Cell Proliferation, 01.01.2019.

Research output: Contribution to journalArticle

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