Spatial analysis of a microbead using surface plasmon resonance coupled fluorescence

Soon Joon Yoon, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Surface plasmon resonance (SPR) coupled fluorescence uses an evanescent electromagnetic field to excite fluorophores in the vicinity of surface. We investigated the influence of enhanced evanescent fields at SPR on the induced fluorescence intensity. The system of this study is based on angle scanning with a half-cylinder prism (SF10) and dual motorized rotation stages to observe the correlation between the evanescent fields and fluorescent intensity of microbeads. With this system, emission from fluorophores only exists in close proximity to the surface of the microbead. The results show that evanescent fields produced at SPR provide more sensitive fluorescence images compared to those measured at a total internal reflection angle.

Original languageEnglish
Title of host publicationPlasmonics in Biology and Medicine IV
Volume6450
DOIs
Publication statusPublished - 2007 Apr 30
EventPlasmonics in Biology and Medicine IV - San Jose, CA, United States
Duration: 2007 Jan 232007 Jan 23

Other

OtherPlasmonics in Biology and Medicine IV
CountryUnited States
CitySan Jose, CA
Period07/1/2307/1/23

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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