Abstract
Surface plasmon resonance (SPR) coupled fluorescence uses an evanescent electromagnetic field to excite fluorophores in the vicinity of surface. We investigated the influence of enhanced evanescent fields at SPR on the induced fluorescence intensity. The system of this study is based on angle scanning with a half-cylinder prism (SF10) and dual motorized rotation stages to observe the correlation between the evanescent fields and fluorescent intensity of microbeads. With this system, emission from fluorophores only exists in close proximity to the surface of the microbead. The results show that evanescent fields produced at SPR provide more sensitive fluorescence images compared to those measured at a total internal reflection angle.
| Original language | English |
|---|---|
| Title of host publication | Plasmonics in Biology and Medicine IV |
| Volume | 6450 |
| DOIs | |
| Publication status | Published - 2007 Apr 30 |
| Event | Plasmonics in Biology and Medicine IV - San Jose, CA, United States Duration: 2007 Jan 23 → 2007 Jan 23 |
Other
| Other | Plasmonics in Biology and Medicine IV |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 07/1/23 → 07/1/23 |
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
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Cite this
Yoon, S. J., & Kim, D. (2007). Spatial analysis of a microbead using surface plasmon resonance coupled fluorescence. In Plasmonics in Biology and Medicine IV (Vol. 6450). [64500O] https://doi.org/10.1117/12.700343