Spatial analysis of a microbead using surface plasmon resonance coupled fluorescence

Soon Joon Yoon; Donghyun Kim

doi:10.1117/12.700343

Spatial analysis of a microbead using surface plasmon resonance coupled fluorescence

Soon Joon Yoon, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Surface plasmon resonance (SPR) coupled fluorescence uses an evanescent electromagnetic field to excite fluorophores in the vicinity of surface. We investigated the influence of enhanced evanescent fields at SPR on the induced fluorescence intensity. The system of this study is based on angle scanning with a half-cylinder prism (SF10) and dual motorized rotation stages to observe the correlation between the evanescent fields and fluorescent intensity of microbeads. With this system, emission from fluorophores only exists in close proximity to the surface of the microbead. The results show that evanescent fields produced at SPR provide more sensitive fluorescence images compared to those measured at a total internal reflection angle.

Original language	English
Title of host publication	Plasmonics in Biology and Medicine IV
Volume	6450
DOIs	https://doi.org/10.1117/12.700343
Publication status	Published - 2007 Apr 30
Event	Plasmonics in Biology and Medicine IV - San Jose, CA, United States Duration: 2007 Jan 23 → 2007 Jan 23

Other

Other	Plasmonics in Biology and Medicine IV
Country	United States
City	San Jose, CA
Period	07/1/23 → 07/1/23

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All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Cite this

Yoon, S. J., & Kim, D. (2007). Spatial analysis of a microbead using surface plasmon resonance coupled fluorescence. In Plasmonics in Biology and Medicine IV (Vol. 6450). [64500O] https://doi.org/10.1117/12.700343

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AB - Surface plasmon resonance (SPR) coupled fluorescence uses an evanescent electromagnetic field to excite fluorophores in the vicinity of surface. We investigated the influence of enhanced evanescent fields at SPR on the induced fluorescence intensity. The system of this study is based on angle scanning with a half-cylinder prism (SF10) and dual motorized rotation stages to observe the correlation between the evanescent fields and fluorescent intensity of microbeads. With this system, emission from fluorophores only exists in close proximity to the surface of the microbead. The results show that evanescent fields produced at SPR provide more sensitive fluorescence images compared to those measured at a total internal reflection angle.

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10.1117/12.700343