Spatially controlled sonoporation of prostate cancer cells via ultrasound activated microbubble cavitation

P. A. Prentice, D. McLean, A. Cuschieri, K. Dholakia, P. A. Campbell

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

Cells that are exposed to ultrasonic (US) energy, in the presence of ultrasound contrast agent microbubbles, may experience enhanced membrane permeability. If the effective dose of US exceeds some threshold, then cell lysis can result (lethal sonoporation), however for lower doses a transient enhancement of membrane permeability occurs (reversible or non lethal sonoporation). The merits of each mode are clear: lethal sonoporation constitutes a significant tumour therapy weapon, whilst its less intrusive counterpart, reversible sonoporation, represents an effective non-invasive targeted drug delivery technique. Until now, the mechanism of the dynamic interaction between microbubbles and cells has remained unknown. Moreover pores, which are the presumed mode of permeabilization have not been observed in a convincing fashion. We will demonstrate, for the first time, how an innovative hybridization of holographic optical trapping technology, together with the application of MHz pulsed US energy and subsequent high resolution observation using atomic force microscopy has been used to elucidate the fundamental mode for membrane permeabilization during sonoporation.

Original languageEnglish
Title of host publication2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Pages158-159
Number of pages2
DOIs
Publication statusPublished - 2005 Dec 1
Event2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology - Oahu, HI, United States
Duration: 2005 May 122005 May 15

Publication series

Name2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Volume2005

Conference

Conference2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
CountryUnited States
CityOahu, HI
Period05/5/1205/5/15

Fingerprint

Cavitation
Ultrasonics
Cells
Membranes
Tumors
Atomic force microscopy

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Prentice, P. A., McLean, D., Cuschieri, A., Dholakia, K., & Campbell, P. A. (2005). Spatially controlled sonoporation of prostate cancer cells via ultrasound activated microbubble cavitation. In 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology (pp. 158-159). [1548412] (2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology; Vol. 2005). https://doi.org/10.1109/MMB.2005.1548412
Prentice, P. A. ; McLean, D. ; Cuschieri, A. ; Dholakia, K. ; Campbell, P. A. / Spatially controlled sonoporation of prostate cancer cells via ultrasound activated microbubble cavitation. 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology. 2005. pp. 158-159 (2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology).
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Prentice, PA, McLean, D, Cuschieri, A, Dholakia, K & Campbell, PA 2005, Spatially controlled sonoporation of prostate cancer cells via ultrasound activated microbubble cavitation. in 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology., 1548412, 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology, vol. 2005, pp. 158-159, 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology, Oahu, HI, United States, 05/5/12. https://doi.org/10.1109/MMB.2005.1548412

Spatially controlled sonoporation of prostate cancer cells via ultrasound activated microbubble cavitation. / Prentice, P. A.; McLean, D.; Cuschieri, A.; Dholakia, K.; Campbell, P. A.

2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology. 2005. p. 158-159 1548412 (2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology; Vol. 2005).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Prentice PA, McLean D, Cuschieri A, Dholakia K, Campbell PA. Spatially controlled sonoporation of prostate cancer cells via ultrasound activated microbubble cavitation. In 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology. 2005. p. 158-159. 1548412. (2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology). https://doi.org/10.1109/MMB.2005.1548412