Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Yoshihiko Arita, Maria Leilani Torres-Mapa, Woei Ming Lee, Tomáš Čižmár, Paul Campbell, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

Original languageEnglish
Article number093702
JournalApplied Physics Letters
Volume98
Issue number9
DOIs
Publication statusPublished - 2011 Feb 28

Fingerprint

genes
breakdown
nanoparticles
cells
lasers
energy requirements
plasmids
light modulators
cavitation flow
shear stress
deoxyribonucleic acid
microscopes
membranes
pulses

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

Cite this

Arita, Y., Torres-Mapa, M. L., Lee, W. M., Čižmár, T., Campbell, P., Gunn-Moore, F. J., & Dholakia, K. (2011). Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles. Applied Physics Letters, 98(9), [093702]. https://doi.org/10.1063/1.3554415
Arita, Yoshihiko ; Torres-Mapa, Maria Leilani ; Lee, Woei Ming ; Čižmár, Tomáš ; Campbell, Paul ; Gunn-Moore, Frank J. ; Dholakia, Kishan. / Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles. In: Applied Physics Letters. 2011 ; Vol. 98, No. 9.
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Arita, Y, Torres-Mapa, ML, Lee, WM, Čižmár, T, Campbell, P, Gunn-Moore, FJ & Dholakia, K 2011, 'Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles', Applied Physics Letters, vol. 98, no. 9, 093702. https://doi.org/10.1063/1.3554415

Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles. / Arita, Yoshihiko; Torres-Mapa, Maria Leilani; Lee, Woei Ming; Čižmár, Tomáš; Campbell, Paul; Gunn-Moore, Frank J.; Dholakia, Kishan.

In: Applied Physics Letters, Vol. 98, No. 9, 093702, 28.02.2011.

Research output: Contribution to journalArticle

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Arita Y, Torres-Mapa ML, Lee WM, Čižmár T, Campbell P, Gunn-Moore FJ et al. Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles. Applied Physics Letters. 2011 Feb 28;98(9). 093702. https://doi.org/10.1063/1.3554415