Abstract
We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.
| Original language | English |
|---|---|
| Article number | 093702 |
| Journal | Applied Physics Letters |
| Volume | 98 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2011 Feb 28 |
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All Science Journal Classification (ASJC) codes
- Physics and Astronomy (miscellaneous)
Cite this
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Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles. / Arita, Yoshihiko; Torres-Mapa, Maria Leilani; Lee, Woei Ming; Čižmár, Tomáš; Campbell, Paul; Gunn-Moore, Frank J.; Dholakia, Kishan.
In: Applied Physics Letters, Vol. 98, No. 9, 093702, 28.02.2011.Research output: Contribution to journal › Article
TY - JOUR
T1 - Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
AU - Arita, Yoshihiko
AU - Torres-Mapa, Maria Leilani
AU - Lee, Woei Ming
AU - Čižmár, Tomáš
AU - Campbell, Paul
AU - Gunn-Moore, Frank J.
AU - Dholakia, Kishan
PY - 2011/2/28
Y1 - 2011/2/28
N2 - We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.
AB - We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.
UR - http://www.scopus.com/inward/record.url?scp=79952407617&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952407617&partnerID=8YFLogxK
U2 - 10.1063/1.3554415
DO - 10.1063/1.3554415
M3 - Article
AN - SCOPUS:79952407617
VL - 98
JO - Applied Physics Letters
JF - Applied Physics Letters
SN - 0003-6951
IS - 9
M1 - 093702
ER -