Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Yoshihiko Arita, Maria Leilani Torres-Mapa, Woei Ming Lee, Tomáš Čižmár, Paul Campbell, Frank J. Gunn-Moore, Kishan Dholakia

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Abstract

We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

Original languageEnglish
Article number093702
JournalApplied Physics Letters
Volume98
Issue number9
DOIs
Publication statusPublished - 2011 Feb 28

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

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  • Cite this

    Arita, Y., Torres-Mapa, M. L., Lee, W. M., Čižmár, T., Campbell, P., Gunn-Moore, F. J., & Dholakia, K. (2011). Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles. Applied Physics Letters, 98(9), [093702]. https://doi.org/10.1063/1.3554415