We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.
Bibliographical noteFunding Information:
We thank the UK EPSRC for funding. K.D. is a Royal Society Wolfson-Merit Award Holder. P.C. acknowledges the MRC for a Discipline Hopping Fellowship and Milstein Award. M.L.T.-M. acknowledges the support of a SUPA Prize Studentship.
All Science Journal Classification (ASJC) codes
- Physics and Astronomy (miscellaneous)