Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Yoshihiko Arita, Maria Leilani Torres-Mapa, Woei Ming Lee, Tomáš Čižmár, Paul Campbell, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)


We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

Original languageEnglish
Article number093702
JournalApplied Physics Letters
Issue number9
Publication statusPublished - 2011 Feb 28

Bibliographical note

Funding Information:
We thank the UK EPSRC for funding. K.D. is a Royal Society Wolfson-Merit Award Holder. P.C. acknowledges the MRC for a Discipline Hopping Fellowship and Milstein Award. M.L.T.-M. acknowledges the support of a SUPA Prize Studentship.

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)


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