Abstract
An efficient method for specifically determining the viability of endothelial cells (EC) from cells dissociated from the human saphenous vein was investigated. Three different methods, trypan blue staining assay, [3H]-proline incorporation assay, and flow cytometry (FCM), combined with the fluorescein isothiocyanate conjugated with Griffonia simplicifolia agglutins (GS1-FITC)/propidium iodide (PI) double staining, were used. Both trypan blue staining and [3H] proline incorporation assays demonstrated less sensitivity to determine viability of EC differentially from the other cells. FITC-GS1 showed prominent binding to the vascular EC and could be counted by FCM including PI on dead cells. Following the cryopreservation process, the GS1-FITC/PI FCM analytical method was adopted to test simultaneously the viability of whole cells and EC from the same tissue, human saphenous veins, and mongrel dogs' femoral veins after harvesting, antibiotic solution treatment, and thawing. The viability of the whole cells from veins decreased with a significant difference (p < 0.05) from that of EC after thawing.
| Original language | English |
|---|---|
| Pages (from-to) | 829-833 |
| Number of pages | 5 |
| Journal | Artificial Organs |
| Volume | 24 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2000 Nov 13 |
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All Science Journal Classification (ASJC) codes
- Bioengineering
- Medicine (miscellaneous)
- Biomaterials
- Biomedical Engineering
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Specific determination of endothelial cell viability in the whole cell fraction from cryopreserved canine femoral veins using flow cytometry. / Park, Jong Chul; Sung, Hak Joon; Lee, Dong Hee; Park, Young Hwan; Cho, Bum Koo; Suh, Hwal.
In: Artificial Organs, Vol. 24, No. 10, 13.11.2000, p. 829-833.Research output: Contribution to journal › Article
TY - JOUR
T1 - Specific determination of endothelial cell viability in the whole cell fraction from cryopreserved canine femoral veins using flow cytometry
AU - Park, Jong Chul
AU - Sung, Hak Joon
AU - Lee, Dong Hee
AU - Park, Young Hwan
AU - Cho, Bum Koo
AU - Suh, Hwal
PY - 2000/11/13
Y1 - 2000/11/13
N2 - An efficient method for specifically determining the viability of endothelial cells (EC) from cells dissociated from the human saphenous vein was investigated. Three different methods, trypan blue staining assay, [3H]-proline incorporation assay, and flow cytometry (FCM), combined with the fluorescein isothiocyanate conjugated with Griffonia simplicifolia agglutins (GS1-FITC)/propidium iodide (PI) double staining, were used. Both trypan blue staining and [3H] proline incorporation assays demonstrated less sensitivity to determine viability of EC differentially from the other cells. FITC-GS1 showed prominent binding to the vascular EC and could be counted by FCM including PI on dead cells. Following the cryopreservation process, the GS1-FITC/PI FCM analytical method was adopted to test simultaneously the viability of whole cells and EC from the same tissue, human saphenous veins, and mongrel dogs' femoral veins after harvesting, antibiotic solution treatment, and thawing. The viability of the whole cells from veins decreased with a significant difference (p < 0.05) from that of EC after thawing.
AB - An efficient method for specifically determining the viability of endothelial cells (EC) from cells dissociated from the human saphenous vein was investigated. Three different methods, trypan blue staining assay, [3H]-proline incorporation assay, and flow cytometry (FCM), combined with the fluorescein isothiocyanate conjugated with Griffonia simplicifolia agglutins (GS1-FITC)/propidium iodide (PI) double staining, were used. Both trypan blue staining and [3H] proline incorporation assays demonstrated less sensitivity to determine viability of EC differentially from the other cells. FITC-GS1 showed prominent binding to the vascular EC and could be counted by FCM including PI on dead cells. Following the cryopreservation process, the GS1-FITC/PI FCM analytical method was adopted to test simultaneously the viability of whole cells and EC from the same tissue, human saphenous veins, and mongrel dogs' femoral veins after harvesting, antibiotic solution treatment, and thawing. The viability of the whole cells from veins decreased with a significant difference (p < 0.05) from that of EC after thawing.
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UR - http://www.scopus.com/inward/citedby.url?scp=0033764721&partnerID=8YFLogxK
U2 - 10.1046/j.1525-1594.2000.06514-2.x
DO - 10.1046/j.1525-1594.2000.06514-2.x
M3 - Article
C2 - 11091174
AN - SCOPUS:0033764721
VL - 24
SP - 829
EP - 833
JO - Artificial Organs
JF - Artificial Organs
SN - 0160-564X
IS - 10
ER -