Specific IgE measurement using AdvanSure® system: Comparison of detection performance with ImmunoCAP® system in Korean allergy patients

Jae Hyun Lee, Kyung Hee Park, Hyon Suk Kim, Kyung Won Kim, Myung Hyun Sohn, Chang Hoon Kim, Joo Shil Lee, Chein Soo Hong, Jung Won Park

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: AdvanSure enzyme immunoassay (EIA) system is a recently developed multiple allergen screen test with specific immunoglobulin E (sIgE) detection assay, while ImmunoCAP fluorescent EIA (FEIA) has been widely used for sIgE detection. There has been no inter-assay comparison data for these two systems. We determined the detection performance of AdvanSure system compared to that of ImmunoCAP. Methods: We performed an inter-method comparison using sera from 199 Korean allergy patients, including asthma (39.7%), allergic rhinitis (54.8%), atopic dermatitis (36.2%) and food allergies (21.6%). We compared the sIgE detection performance for nine major inhalant and four food allergens. Results: 950 paired assay results were analyzed. Most allergen sIgE results showed above 0.5 intraclass correlation coefficient except Blattella germanica, alternaria and mugwort allergen. Intermethod comparison results showed multiple differences in a few allergens. The inter-method concordance was moderate to substantial for most allergens (κ = 0.528-0.778, p< 0.001), except for cat dander. Conclusion: AdvanSure system showed a good detection performance compared with ImmunoCAP in correlation and agreement in Korean allergy patients. However, in terms of differences in the methodologies used by these two systems, careful clinical correlation is needed for interpretation of AdvanSure EIA results.

Original languageEnglish
Pages (from-to)914-919
Number of pages6
JournalClinica Chimica Acta
Volume413
Issue number9-10
DOIs
Publication statusPublished - 2012 May 18

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Allergies
Allergens
Immunoglobulin E
Hypersensitivity
Immunoenzyme Techniques
Assays
Enzymes
Dander
Artemisia
Alternaria
Food Hypersensitivity
Atopic Dermatitis
Information Systems
Cats
Asthma
Food
Serum

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Lee, Jae Hyun ; Park, Kyung Hee ; Kim, Hyon Suk ; Kim, Kyung Won ; Sohn, Myung Hyun ; Kim, Chang Hoon ; Lee, Joo Shil ; Hong, Chein Soo ; Park, Jung Won. / Specific IgE measurement using AdvanSure® system : Comparison of detection performance with ImmunoCAP® system in Korean allergy patients. In: Clinica Chimica Acta. 2012 ; Vol. 413, No. 9-10. pp. 914-919.
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abstract = "Background: AdvanSure enzyme immunoassay (EIA) system is a recently developed multiple allergen screen test with specific immunoglobulin E (sIgE) detection assay, while ImmunoCAP fluorescent EIA (FEIA) has been widely used for sIgE detection. There has been no inter-assay comparison data for these two systems. We determined the detection performance of AdvanSure system compared to that of ImmunoCAP. Methods: We performed an inter-method comparison using sera from 199 Korean allergy patients, including asthma (39.7{\%}), allergic rhinitis (54.8{\%}), atopic dermatitis (36.2{\%}) and food allergies (21.6{\%}). We compared the sIgE detection performance for nine major inhalant and four food allergens. Results: 950 paired assay results were analyzed. Most allergen sIgE results showed above 0.5 intraclass correlation coefficient except Blattella germanica, alternaria and mugwort allergen. Intermethod comparison results showed multiple differences in a few allergens. The inter-method concordance was moderate to substantial for most allergens (κ = 0.528-0.778, p< 0.001), except for cat dander. Conclusion: AdvanSure system showed a good detection performance compared with ImmunoCAP in correlation and agreement in Korean allergy patients. However, in terms of differences in the methodologies used by these two systems, careful clinical correlation is needed for interpretation of AdvanSure EIA results.",
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Specific IgE measurement using AdvanSure® system : Comparison of detection performance with ImmunoCAP® system in Korean allergy patients. / Lee, Jae Hyun; Park, Kyung Hee; Kim, Hyon Suk; Kim, Kyung Won; Sohn, Myung Hyun; Kim, Chang Hoon; Lee, Joo Shil; Hong, Chein Soo; Park, Jung Won.

In: Clinica Chimica Acta, Vol. 413, No. 9-10, 18.05.2012, p. 914-919.

Research output: Contribution to journalArticle

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T1 - Specific IgE measurement using AdvanSure® system

T2 - Comparison of detection performance with ImmunoCAP® system in Korean allergy patients

AU - Lee, Jae Hyun

AU - Park, Kyung Hee

AU - Kim, Hyon Suk

AU - Kim, Kyung Won

AU - Sohn, Myung Hyun

AU - Kim, Chang Hoon

AU - Lee, Joo Shil

AU - Hong, Chein Soo

AU - Park, Jung Won

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N2 - Background: AdvanSure enzyme immunoassay (EIA) system is a recently developed multiple allergen screen test with specific immunoglobulin E (sIgE) detection assay, while ImmunoCAP fluorescent EIA (FEIA) has been widely used for sIgE detection. There has been no inter-assay comparison data for these two systems. We determined the detection performance of AdvanSure system compared to that of ImmunoCAP. Methods: We performed an inter-method comparison using sera from 199 Korean allergy patients, including asthma (39.7%), allergic rhinitis (54.8%), atopic dermatitis (36.2%) and food allergies (21.6%). We compared the sIgE detection performance for nine major inhalant and four food allergens. Results: 950 paired assay results were analyzed. Most allergen sIgE results showed above 0.5 intraclass correlation coefficient except Blattella germanica, alternaria and mugwort allergen. Intermethod comparison results showed multiple differences in a few allergens. The inter-method concordance was moderate to substantial for most allergens (κ = 0.528-0.778, p< 0.001), except for cat dander. Conclusion: AdvanSure system showed a good detection performance compared with ImmunoCAP in correlation and agreement in Korean allergy patients. However, in terms of differences in the methodologies used by these two systems, careful clinical correlation is needed for interpretation of AdvanSure EIA results.

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