TY - JOUR
T1 - Specific IgE measurement using AdvanSure® system
T2 - Comparison of detection performance with ImmunoCAP® system in Korean allergy patients
AU - Lee, Jae Hyun
AU - Park, Kyung Hee
AU - Kim, Hyon Suk
AU - Kim, Kyung Won
AU - Sohn, Myung Hyun
AU - Kim, Chang Hoon
AU - Lee, Joo Shil
AU - Hong, Chein Soo
AU - Park, Jung Won
PY - 2012/5/18
Y1 - 2012/5/18
N2 - Background: AdvanSure enzyme immunoassay (EIA) system is a recently developed multiple allergen screen test with specific immunoglobulin E (sIgE) detection assay, while ImmunoCAP fluorescent EIA (FEIA) has been widely used for sIgE detection. There has been no inter-assay comparison data for these two systems. We determined the detection performance of AdvanSure system compared to that of ImmunoCAP. Methods: We performed an inter-method comparison using sera from 199 Korean allergy patients, including asthma (39.7%), allergic rhinitis (54.8%), atopic dermatitis (36.2%) and food allergies (21.6%). We compared the sIgE detection performance for nine major inhalant and four food allergens. Results: 950 paired assay results were analyzed. Most allergen sIgE results showed above 0.5 intraclass correlation coefficient except Blattella germanica, alternaria and mugwort allergen. Intermethod comparison results showed multiple differences in a few allergens. The inter-method concordance was moderate to substantial for most allergens (κ = 0.528-0.778, p< 0.001), except for cat dander. Conclusion: AdvanSure system showed a good detection performance compared with ImmunoCAP in correlation and agreement in Korean allergy patients. However, in terms of differences in the methodologies used by these two systems, careful clinical correlation is needed for interpretation of AdvanSure EIA results.
AB - Background: AdvanSure enzyme immunoassay (EIA) system is a recently developed multiple allergen screen test with specific immunoglobulin E (sIgE) detection assay, while ImmunoCAP fluorescent EIA (FEIA) has been widely used for sIgE detection. There has been no inter-assay comparison data for these two systems. We determined the detection performance of AdvanSure system compared to that of ImmunoCAP. Methods: We performed an inter-method comparison using sera from 199 Korean allergy patients, including asthma (39.7%), allergic rhinitis (54.8%), atopic dermatitis (36.2%) and food allergies (21.6%). We compared the sIgE detection performance for nine major inhalant and four food allergens. Results: 950 paired assay results were analyzed. Most allergen sIgE results showed above 0.5 intraclass correlation coefficient except Blattella germanica, alternaria and mugwort allergen. Intermethod comparison results showed multiple differences in a few allergens. The inter-method concordance was moderate to substantial for most allergens (κ = 0.528-0.778, p< 0.001), except for cat dander. Conclusion: AdvanSure system showed a good detection performance compared with ImmunoCAP in correlation and agreement in Korean allergy patients. However, in terms of differences in the methodologies used by these two systems, careful clinical correlation is needed for interpretation of AdvanSure EIA results.
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U2 - 10.1016/j.cca.2012.02.018
DO - 10.1016/j.cca.2012.02.018
M3 - Article
C2 - 22394454
AN - SCOPUS:84862790136
VL - 413
SP - 914
EP - 919
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 9-10
ER -