Specific IgE measurement using AdvanSure® system: Comparison of detection performance with ImmunoCAP® system in Korean allergy patients

Jae Hyun Lee, Kyung Hee Park, Hyon Suk Kim, Kyung Won Kim, Myung Hyun Sohn, Chang Hoon Kim, Joo Shil Lee, Chein Soo Hong, Jung Won Park

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: AdvanSure enzyme immunoassay (EIA) system is a recently developed multiple allergen screen test with specific immunoglobulin E (sIgE) detection assay, while ImmunoCAP fluorescent EIA (FEIA) has been widely used for sIgE detection. There has been no inter-assay comparison data for these two systems. We determined the detection performance of AdvanSure system compared to that of ImmunoCAP. Methods: We performed an inter-method comparison using sera from 199 Korean allergy patients, including asthma (39.7%), allergic rhinitis (54.8%), atopic dermatitis (36.2%) and food allergies (21.6%). We compared the sIgE detection performance for nine major inhalant and four food allergens. Results: 950 paired assay results were analyzed. Most allergen sIgE results showed above 0.5 intraclass correlation coefficient except Blattella germanica, alternaria and mugwort allergen. Intermethod comparison results showed multiple differences in a few allergens. The inter-method concordance was moderate to substantial for most allergens (κ = 0.528-0.778, p< 0.001), except for cat dander. Conclusion: AdvanSure system showed a good detection performance compared with ImmunoCAP in correlation and agreement in Korean allergy patients. However, in terms of differences in the methodologies used by these two systems, careful clinical correlation is needed for interpretation of AdvanSure EIA results.

Original languageEnglish
Pages (from-to)914-919
Number of pages6
JournalClinica Chimica Acta
Volume413
Issue number9-10
DOIs
Publication statusPublished - 2012 May 18

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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