Spectral-domain optical coherence phase and multiphoton microscopy

Chulmin Joo, Ki Hean Kim, Johannes F. De Boer

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Abstract

We describe simultaneous quantitative phase contrast and multiphoton fluorescence imaging by combined spectral-domain optical coherence phase and multiphoton microscopy. The instrument employs two light sources for efficient optical coherence microscopic and multiphoton imaging and can generate structural and functional images of transparent specimens in the epidirection. Phase contrast imaging exhibits spatial and temporal phase stability in the subnanometer range. We also demonstrate the visualization of actin filaments in a fixed cell specimen, which is confirmed by simultaneous multiphoton fluorescence imaging.

Original languageEnglish
Pages (from-to)623-625
Number of pages3
JournalOptics Letters
Volume32
Issue number6
DOIs
Publication statusPublished - 2007 Mar 15

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All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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