Spectral-domain optical coherence phase microscopy: A new optical imaging tool for quantitative biology

Suho Ryu, Chulmin Joo

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Quantifying depth-resolved nanoscale dynamics in biological specimens (e.g., cells) provides a new dimension of study, which may serve as a means of phenotypically differentiating cellular responses to external stimuli and measuring molecular interactions in diverse platforms. Spectral-domain optical coherence phase microscopy (SD-OCPM) represents a novel optical microscopy technique, capable of measuring optical path-length changes inside the specimens with nanometer-level sensitivity. In this chapter, we describe operating principle and implementation of SD-OCPM. Theoretical discussion of SD-OCPM phase stability and spatial resolution will be presented, along with representative applications of SD-OCPM for quantitative biology. Future outlook and potential applications of SD-OCPM will also be discussed.

Original languageEnglish
Title of host publicationHandbook of Photonics for Biomedical Engineering
PublisherSpringer Netherlands
Pages325-350
Number of pages26
ISBN (Electronic)9789400750524
ISBN (Print)9789400750517
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

phase coherence
Optical Imaging
biology
Microscopy
Microscopic examination
microscopy
Imaging techniques
Phase stability
Molecular interactions
Optical microscopy
molecular interactions
optical paths
stimuli
platforms
spatial resolution
sensitivity
cells

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Physics and Astronomy(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ryu, S., & Joo, C. (2017). Spectral-domain optical coherence phase microscopy: A new optical imaging tool for quantitative biology. In Handbook of Photonics for Biomedical Engineering (pp. 325-350). Springer Netherlands. https://doi.org/10.1007/978-94-007-5052-4_25
Ryu, Suho ; Joo, Chulmin. / Spectral-domain optical coherence phase microscopy : A new optical imaging tool for quantitative biology. Handbook of Photonics for Biomedical Engineering. Springer Netherlands, 2017. pp. 325-350
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Spectral-domain optical coherence phase microscopy : A new optical imaging tool for quantitative biology. / Ryu, Suho; Joo, Chulmin.

Handbook of Photonics for Biomedical Engineering. Springer Netherlands, 2017. p. 325-350.

Research output: Chapter in Book/Report/Conference proceedingChapter

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