Spectral, In Vitro Biological, Engine and Emission Performances of Biodiesel Production from Chlorella protothecoides: A Sustainable Renewable Energy Source

Sundaram Arvindnarayan, Sutha Shobana, Jeyaprakash Dharmaraja, Dinh Duc Nguyen, Soon Woong Chang, A. E. Atabani, Gopalakrishnan Kumar, Kandasamy K.Sivagnana Prabhu

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Abstract: In this research, microalgae species, Chlorella protothecoides was selected for biodiesel production due to its ability to produce large amount of hydrocarbons and oils with high lipid composition. The extracted bio-oil was characterized systematically by proximate, ultimate, spectral (FT-IR, UV–vis., GC–Mass, 1H NMR and 13C NMR) and thermogravimetric (TG/DTA) techniques. The fuel characterization of the bio-oil was evaluated using standard methods. The bio-oil samples were examined for their notable in vitro antimicrobial as well as antioxidant activities. The engine parameters unlike brake specific fuel consumption and brake thermal efficiency for three fuel samples namely diesel (B100), microalga biodiesel 20% blend (CB20) and microalga biodiesel 50% blend (CB50) along with their emission characteristics towards CO2, NOx, and HCs were measured. Graphic Abstract: Chlorella protothecoides micro alga was selected for bio-oil extraction. The bio-oil extracted was characterized by proximate, elemental, spectral and thermogravimetric techniques and their biodiesel potentiality, the fuel properties were evaluated using standard methods, consequently compared to the standards. In addition, the bio-oil samples were tested for their in vitro antimicrobial and antioxidant activities. The fuel properties show that the microalgae bio-diesel has a cold filter plugging point (CFPP) around –13 °C with 4.5 h oxidation stability. The micro algal oil produces high efficiency (ηbth), low BSFC with lesser CO, (NO)x and hydrocarbons emissions with a single cylinder, water cooled, DI four stroke diesel engines using algae oil blends which is an alternative to diesel engine. Moreover, CB50 blend has a good combustion and emission characteristics when compared to CB20 and B100 fuels. [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)5809-5819
Number of pages11
JournalWaste and Biomass Valorization
Issue number11
Publication statusPublished - 2020 Nov 1

Bibliographical note

Funding Information:
This work was supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (Grant No. 20194110300040 and Grant No. 20173010092470). The authors would also like to acknowledge STIC, CUSAT, Cochin for giving the analytical facilities.

Publisher Copyright:
© 2019, Springer Nature B.V.

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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