Spheroid cell culture on PDMS hydrophobic surfaces and integration into microfludic devices

Patrick Ingram, Maesoon Im, Sean McDermott, Max Wicha, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

We report successful non-adherent spheroid culture of cancer cells on hydrophobic surfaces, formed in polydimethylsiloxane (PDMS), and its extension to high-throughput culture within an integrated microfluidic device. The surface hydrophobicity was characterized by observing the contact angle of deionized water droplets. Various cell types were grown on the hydrophobic designs (circular, triangular, square, and honeycomb), but only the honeycomb hydrophobic design was capable of maintaining hydrophobicity to prevent cell adhesion. With the honeycomb hydrophobic surface, cancer spheroids were grown in suspension starting from single cancer stem cells both in macro-scale wells and in integrated microfluidic microwells.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1539-1541
Number of pages3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Fingerprint

Polydimethylsiloxane
Cell culture
Hydrophobicity
Microfluidics
Deionized water
Cell adhesion
Stem cells
Contact angle
Macros
Cells
Throughput

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Ingram, P., Im, M., McDermott, S., Wicha, M., & Yoon, E. (2011). Spheroid cell culture on PDMS hydrophobic surfaces and integration into microfludic devices. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1539-1541). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).
Ingram, Patrick ; Im, Maesoon ; McDermott, Sean ; Wicha, Max ; Yoon, Euisik. / Spheroid cell culture on PDMS hydrophobic surfaces and integration into microfludic devices. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 1539-1541 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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abstract = "We report successful non-adherent spheroid culture of cancer cells on hydrophobic surfaces, formed in polydimethylsiloxane (PDMS), and its extension to high-throughput culture within an integrated microfluidic device. The surface hydrophobicity was characterized by observing the contact angle of deionized water droplets. Various cell types were grown on the hydrophobic designs (circular, triangular, square, and honeycomb), but only the honeycomb hydrophobic design was capable of maintaining hydrophobicity to prevent cell adhesion. With the honeycomb hydrophobic surface, cancer spheroids were grown in suspension starting from single cancer stem cells both in macro-scale wells and in integrated microfluidic microwells.",
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Ingram, P, Im, M, McDermott, S, Wicha, M & Yoon, E 2011, Spheroid cell culture on PDMS hydrophobic surfaces and integration into microfludic devices. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 3, pp. 1539-1541, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11/10/2.

Spheroid cell culture on PDMS hydrophobic surfaces and integration into microfludic devices. / Ingram, Patrick; Im, Maesoon; McDermott, Sean; Wicha, Max; Yoon, Euisik.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1539-1541 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - We report successful non-adherent spheroid culture of cancer cells on hydrophobic surfaces, formed in polydimethylsiloxane (PDMS), and its extension to high-throughput culture within an integrated microfluidic device. The surface hydrophobicity was characterized by observing the contact angle of deionized water droplets. Various cell types were grown on the hydrophobic designs (circular, triangular, square, and honeycomb), but only the honeycomb hydrophobic design was capable of maintaining hydrophobicity to prevent cell adhesion. With the honeycomb hydrophobic surface, cancer spheroids were grown in suspension starting from single cancer stem cells both in macro-scale wells and in integrated microfluidic microwells.

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Ingram P, Im M, McDermott S, Wicha M, Yoon E. Spheroid cell culture on PDMS hydrophobic surfaces and integration into microfludic devices. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1539-1541. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).