Spread of CTX-M-type extended-spectrum β-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital

Sang Guk Lee, Seokhoon Jeong, Hyukmin Lee, Chang Ki Kim, Yangsoon Lee, Eunmi Koh, Yunsop Chong, Kyungwon Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This study was to determine the prevalence and characteristics of CTX-M-type extended-spectrum β-lactamases (ESBLs) in nonduplicate Escherichia coli (n = 760) and Klebsiella pneumoniae (n = 379) bloodstream isolates collected during January 2005 to October 2007 at a university hospital (2000 beds) in Seoul, Korea. Antimicrobial susceptibilities were determined by disk diffusion and agar dilution methods. The double-disk synergy test detected ESBLs in 8.7% (66/760) of E. coli and 11.3% (43/379) of K. pneumoniae isolates. Polymerase chain reaction detected blaCTX-M in 60/66 (90.9%) E. coli and 9/43 (20.9%) K. pneumoniae isolates with the ESBL phenotype. CTX-M-14 was the most common type of CTX-M ESBLs in both E. coli (n = 32) and K. pneumoniae (n = 6). CTX-M-15 was the 2nd most common type of CTX-M ESBLs in E. coli (n = 22), but it was not detected in K. pneumoniae. In addition, CTX-M-24 (n = 2), CTX-M-65 (n = 2), CTX-M-27 (n = 1), and CTX-M-32 (n = 1) were detected for the 1st time in Korea.

Original languageEnglish
Pages (from-to)76-80
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume63
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

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Klebsiella pneumoniae
Escherichia coli
Korea
Agar
Phenotype
Polymerase Chain Reaction
beta-lactamase CTX-2

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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title = "Spread of CTX-M-type extended-spectrum β-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital",
abstract = "This study was to determine the prevalence and characteristics of CTX-M-type extended-spectrum β-lactamases (ESBLs) in nonduplicate Escherichia coli (n = 760) and Klebsiella pneumoniae (n = 379) bloodstream isolates collected during January 2005 to October 2007 at a university hospital (2000 beds) in Seoul, Korea. Antimicrobial susceptibilities were determined by disk diffusion and agar dilution methods. The double-disk synergy test detected ESBLs in 8.7{\%} (66/760) of E. coli and 11.3{\%} (43/379) of K. pneumoniae isolates. Polymerase chain reaction detected blaCTX-M in 60/66 (90.9{\%}) E. coli and 9/43 (20.9{\%}) K. pneumoniae isolates with the ESBL phenotype. CTX-M-14 was the most common type of CTX-M ESBLs in both E. coli (n = 32) and K. pneumoniae (n = 6). CTX-M-15 was the 2nd most common type of CTX-M ESBLs in E. coli (n = 22), but it was not detected in K. pneumoniae. In addition, CTX-M-24 (n = 2), CTX-M-65 (n = 2), CTX-M-27 (n = 1), and CTX-M-32 (n = 1) were detected for the 1st time in Korea.",
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Spread of CTX-M-type extended-spectrum β-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital. / Lee, Sang Guk; Jeong, Seokhoon; Lee, Hyukmin; Kim, Chang Ki; Lee, Yangsoon; Koh, Eunmi; Chong, Yunsop; Lee, Kyungwon.

In: Diagnostic Microbiology and Infectious Disease, Vol. 63, No. 1, 01.01.2009, p. 76-80.

Research output: Contribution to journalArticle

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T1 - Spread of CTX-M-type extended-spectrum β-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital

AU - Lee, Sang Guk

AU - Jeong, Seokhoon

AU - Lee, Hyukmin

AU - Kim, Chang Ki

AU - Lee, Yangsoon

AU - Koh, Eunmi

AU - Chong, Yunsop

AU - Lee, Kyungwon

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AB - This study was to determine the prevalence and characteristics of CTX-M-type extended-spectrum β-lactamases (ESBLs) in nonduplicate Escherichia coli (n = 760) and Klebsiella pneumoniae (n = 379) bloodstream isolates collected during January 2005 to October 2007 at a university hospital (2000 beds) in Seoul, Korea. Antimicrobial susceptibilities were determined by disk diffusion and agar dilution methods. The double-disk synergy test detected ESBLs in 8.7% (66/760) of E. coli and 11.3% (43/379) of K. pneumoniae isolates. Polymerase chain reaction detected blaCTX-M in 60/66 (90.9%) E. coli and 9/43 (20.9%) K. pneumoniae isolates with the ESBL phenotype. CTX-M-14 was the most common type of CTX-M ESBLs in both E. coli (n = 32) and K. pneumoniae (n = 6). CTX-M-15 was the 2nd most common type of CTX-M ESBLs in E. coli (n = 22), but it was not detected in K. pneumoniae. In addition, CTX-M-24 (n = 2), CTX-M-65 (n = 2), CTX-M-27 (n = 1), and CTX-M-32 (n = 1) were detected for the 1st time in Korea.

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