TY - JOUR
T1 - Spread of CTX-M-type extended-spectrum β-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital
AU - Lee, Sang Guk
AU - Jeong, Seok Hoon
AU - Lee, Hyukmin
AU - Kim, Chang Ki
AU - Lee, Yangsoon
AU - Koh, Eunmi
AU - Chong, Yunsop
AU - Lee, Kyungwon
PY - 2009/1
Y1 - 2009/1
N2 - This study was to determine the prevalence and characteristics of CTX-M-type extended-spectrum β-lactamases (ESBLs) in nonduplicate Escherichia coli (n = 760) and Klebsiella pneumoniae (n = 379) bloodstream isolates collected during January 2005 to October 2007 at a university hospital (2000 beds) in Seoul, Korea. Antimicrobial susceptibilities were determined by disk diffusion and agar dilution methods. The double-disk synergy test detected ESBLs in 8.7% (66/760) of E. coli and 11.3% (43/379) of K. pneumoniae isolates. Polymerase chain reaction detected blaCTX-M in 60/66 (90.9%) E. coli and 9/43 (20.9%) K. pneumoniae isolates with the ESBL phenotype. CTX-M-14 was the most common type of CTX-M ESBLs in both E. coli (n = 32) and K. pneumoniae (n = 6). CTX-M-15 was the 2nd most common type of CTX-M ESBLs in E. coli (n = 22), but it was not detected in K. pneumoniae. In addition, CTX-M-24 (n = 2), CTX-M-65 (n = 2), CTX-M-27 (n = 1), and CTX-M-32 (n = 1) were detected for the 1st time in Korea.
AB - This study was to determine the prevalence and characteristics of CTX-M-type extended-spectrum β-lactamases (ESBLs) in nonduplicate Escherichia coli (n = 760) and Klebsiella pneumoniae (n = 379) bloodstream isolates collected during January 2005 to October 2007 at a university hospital (2000 beds) in Seoul, Korea. Antimicrobial susceptibilities were determined by disk diffusion and agar dilution methods. The double-disk synergy test detected ESBLs in 8.7% (66/760) of E. coli and 11.3% (43/379) of K. pneumoniae isolates. Polymerase chain reaction detected blaCTX-M in 60/66 (90.9%) E. coli and 9/43 (20.9%) K. pneumoniae isolates with the ESBL phenotype. CTX-M-14 was the most common type of CTX-M ESBLs in both E. coli (n = 32) and K. pneumoniae (n = 6). CTX-M-15 was the 2nd most common type of CTX-M ESBLs in E. coli (n = 22), but it was not detected in K. pneumoniae. In addition, CTX-M-24 (n = 2), CTX-M-65 (n = 2), CTX-M-27 (n = 1), and CTX-M-32 (n = 1) were detected for the 1st time in Korea.
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U2 - 10.1016/j.diagmicrobio.2008.09.002
DO - 10.1016/j.diagmicrobio.2008.09.002
M3 - Article
C2 - 19073302
AN - SCOPUS:57349089787
SN - 0732-8893
VL - 63
SP - 76
EP - 80
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 1
ER -
