Src family kinase potentiates the activity of nicotinic acetylcholine receptor in rat autonomic ganglion innervating urinary bladder

Na Hyun Kim, Kyu Sang Park, Seung Kuy Cha, Joon Ho Yoon, Byung Il Yeh, Kyou Hoon Han, In Deok Kong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Src family kinases (SFKs), one of the tyrosine kinase groups, are primary regulators of signal transductions that control cellular functions such as cell proliferation, differentiation, survival, metabolism, and other important roles of the cell. One of the crucial functions of SFKs is to regulate the activities of various neuronal channels. In this study, we investigated the modulatory action of SFK on nicotinic acetylcholine receptors (nAChRs) expressed in rat major pelvic ganglion (MPG) neurons innervating the urinary bladder. PP1 and PP2 (5μM), selective Src-kinase inhibitors, attenuated ACh-induced ionic currents and [Ca2+]i transients in MPG neurons, whereas PP3, an inactive analogue, had no effect. Blocking the tyrosine kinase activity of Src kinase by pp60 c-src inhibitory peptide also reduced the ACh-induced currents. Conversely, sodium orthovanadate (200μM), a tyrosine phosphatase inhibitor, significantly augmented the ACh-induced currents. In the kinase assay, the activities of SFKs in MPG neurons were also inhibited by PP2, but not by PP3. These data suggests that SFKs may have a facilitative role on the synaptic transmission in rat pelvic autonomic ganglion.

Original languageEnglish
Pages (from-to)190-195
Number of pages6
JournalNeuroscience Letters
Volume494
Issue number3
DOIs
Publication statusPublished - 2011 May 2

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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