MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processingof primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesisdefective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.
Bibliographical noteFunding Information:
The National Research Foundation of Korea by the Korean Government MEST [2011-0027376 to B.-h.L., 2012R1A1A2008826 to B.-h.L.]; Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ009104 to B.-h.L.]; Sogang University Research [201214003.01 to B.-h.L.]; UNIK research initiative of the Danish Ministry of Science, Technology and Innovation [09-065274 to S.W.Y.]; Chinese Academy of Sciences and National Institutes of Health Grants [R01GM070795 to J.-K.Z., R01GM059138 to J.-K.Z.]; USDA Grant [2008-35100-04518 to J.-K.Z.]; University of California at Riverside Initial Complement Fund and a United States Department of Agriculture hatch fund [CA-R-BPS-7754H to R.L.]. Funding for open access charge: National Research Foundation of Korea by the Korean Government MEST [2012R1A1A2008826 to B.-h.L.]
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