STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis

Samir Ben Chaabane, Renyi Liu, Viswanathan Chinnusamy, Yerim Kwon, Joo Hyuk Park, Seo Yeon Kim, Jian Kang Zhu, Seong Wook Yang, Byeong Ha Lee

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processingof primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesisdefective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.

Original languageEnglish
Pages (from-to)1984-1997
Number of pages14
JournalNucleic acids research
Volume41
Issue number3
DOIs
Publication statusPublished - 2013 Feb 1

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RNA Precursors
MicroRNAs
Arabidopsis
RNA Cap-Binding Proteins
Developmental Genes
Eukaryota
Introns

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Ben Chaabane, S., Liu, R., Chinnusamy, V., Kwon, Y., Park, J. H., Kim, S. Y., ... Lee, B. H. (2013). STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis. Nucleic acids research, 41(3), 1984-1997. https://doi.org/10.1093/nar/gks1309
Ben Chaabane, Samir ; Liu, Renyi ; Chinnusamy, Viswanathan ; Kwon, Yerim ; Park, Joo Hyuk ; Kim, Seo Yeon ; Zhu, Jian Kang ; Yang, Seong Wook ; Lee, Byeong Ha. / STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis. In: Nucleic acids research. 2013 ; Vol. 41, No. 3. pp. 1984-1997.
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Ben Chaabane, S, Liu, R, Chinnusamy, V, Kwon, Y, Park, JH, Kim, SY, Zhu, JK, Yang, SW & Lee, BH 2013, 'STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis', Nucleic acids research, vol. 41, no. 3, pp. 1984-1997. https://doi.org/10.1093/nar/gks1309

STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis. / Ben Chaabane, Samir; Liu, Renyi; Chinnusamy, Viswanathan; Kwon, Yerim; Park, Joo Hyuk; Kim, Seo Yeon; Zhu, Jian Kang; Yang, Seong Wook; Lee, Byeong Ha.

In: Nucleic acids research, Vol. 41, No. 3, 01.02.2013, p. 1984-1997.

Research output: Contribution to journalArticle

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AU - Ben Chaabane, Samir

AU - Liu, Renyi

AU - Chinnusamy, Viswanathan

AU - Kwon, Yerim

AU - Park, Joo Hyuk

AU - Kim, Seo Yeon

AU - Zhu, Jian Kang

AU - Yang, Seong Wook

AU - Lee, Byeong Ha

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N2 - MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processingof primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesisdefective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.

AB - MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processingof primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesisdefective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.

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