STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis

Samir Ben Chaabane, Renyi Liu, Viswanathan Chinnusamy, Yerim Kwon, Joo Hyuk Park, Seo Yeon Kim, Jian Kang Zhu, Seong Wook Yang, Byeong Ha Lee

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63 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processingof primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesisdefective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.

Original languageEnglish
Pages (from-to)1984-1997
Number of pages14
JournalNucleic acids research
Volume41
Issue number3
DOIs
Publication statusPublished - 2013 Feb

All Science Journal Classification (ASJC) codes

  • Genetics

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    Ben Chaabane, S., Liu, R., Chinnusamy, V., Kwon, Y., Park, J. H., Kim, S. Y., Zhu, J. K., Yang, S. W., & Lee, B. H. (2013). STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis. Nucleic acids research, 41(3), 1984-1997. https://doi.org/10.1093/nar/gks1309