Standardization of house dust mite extracts in Korea

Kyoung Yong Jeong, Soo Young Choi, Jae Hyun Lee, In Yong Lee, Taisoon Yong, Joo Shil Lee, Chein Soo Hong, Jungwon Park

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Purpose: House dust mites are the most important cause of respiratory allergy in Korea. Standardization of allergen extracts is essential for improving diagnostics and immunotherapeutics. This study was undertaken to evaluate the allergenicity of standardized house dust mite allergen extracts from Korean house dust mite isolates. Methods: Allergen extracts were prepared from cultured Korean house dust mites (Dermatophagoides farinae and D. pteronyssinus). Allergenic activities of Korean house dust mite extracts were compared to standardized extracts from a company in the United States whose allergen concentrations were expressed as Allergy Units (AUs). Specifically, we compared group 1 and 2 major allergens using two-site enzyme-linked immunosorbent assay (ELISA) kits and an in vivo intradermal test. Results: Major allergen concentrations were 17.0 μg/mg (5.0 μg/mg of Der f 1 and 12.0 μg/mg of Der f 2) for a D. farinae extract and 24.0 μg/mg (11.6 μg/mg of Der p 1 and 12.4 μg/mg of Der p 2) for a D. pteronyssinus extract. Using chloramphenicol (CAP) inhibition assays, AUs were 12.5 AU/μg for a D. farinae extract and 12.8 AU/μg for a D. pteronyssinus extract. Allergenic activities were 3- to 4-fold stronger when assessed by intradermal skin tests for in vivo standardization. Conclusions: Allergen extracts were prepared from Korean house dust mites and the allergenicities of the extracts were estimated using AU measurements. House dust mite extracts prepared in this study could be utilized as a reference material, which will be useful for the development of diagnostic and immunotherapeutic reagents in Korea.

Original languageEnglish
Pages (from-to)346-350
Number of pages5
JournalAllergy, Asthma and Immunology Research
Volume4
Issue number6
DOIs
Publication statusPublished - 2012 Nov 1

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Pyroglyphidae
Korea
Allergens
Hypersensitivity
Dermatophagoides farinae
Intradermal Tests
Dermatophagoides Antigens
Chloramphenicol
Skin Tests
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pulmonary and Respiratory Medicine

Cite this

Jeong, Kyoung Yong ; Choi, Soo Young ; Lee, Jae Hyun ; Lee, In Yong ; Yong, Taisoon ; Lee, Joo Shil ; Hong, Chein Soo ; Park, Jungwon. / Standardization of house dust mite extracts in Korea. In: Allergy, Asthma and Immunology Research. 2012 ; Vol. 4, No. 6. pp. 346-350.
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abstract = "Purpose: House dust mites are the most important cause of respiratory allergy in Korea. Standardization of allergen extracts is essential for improving diagnostics and immunotherapeutics. This study was undertaken to evaluate the allergenicity of standardized house dust mite allergen extracts from Korean house dust mite isolates. Methods: Allergen extracts were prepared from cultured Korean house dust mites (Dermatophagoides farinae and D. pteronyssinus). Allergenic activities of Korean house dust mite extracts were compared to standardized extracts from a company in the United States whose allergen concentrations were expressed as Allergy Units (AUs). Specifically, we compared group 1 and 2 major allergens using two-site enzyme-linked immunosorbent assay (ELISA) kits and an in vivo intradermal test. Results: Major allergen concentrations were 17.0 μg/mg (5.0 μg/mg of Der f 1 and 12.0 μg/mg of Der f 2) for a D. farinae extract and 24.0 μg/mg (11.6 μg/mg of Der p 1 and 12.4 μg/mg of Der p 2) for a D. pteronyssinus extract. Using chloramphenicol (CAP) inhibition assays, AUs were 12.5 AU/μg for a D. farinae extract and 12.8 AU/μg for a D. pteronyssinus extract. Allergenic activities were 3- to 4-fold stronger when assessed by intradermal skin tests for in vivo standardization. Conclusions: Allergen extracts were prepared from Korean house dust mites and the allergenicities of the extracts were estimated using AU measurements. House dust mite extracts prepared in this study could be utilized as a reference material, which will be useful for the development of diagnostic and immunotherapeutic reagents in Korea.",
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Standardization of house dust mite extracts in Korea. / Jeong, Kyoung Yong; Choi, Soo Young; Lee, Jae Hyun; Lee, In Yong; Yong, Taisoon; Lee, Joo Shil; Hong, Chein Soo; Park, Jungwon.

In: Allergy, Asthma and Immunology Research, Vol. 4, No. 6, 01.11.2012, p. 346-350.

Research output: Contribution to journalArticle

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