State-of-the-art technologies for continuous high-rate biohydrogen production

Jong Hun Park, K. Chandrasekhar, Byong Hun Jeon, Min Jang, Yang Liu, Sang Hyoun Kim

Research output: Contribution to journalReview articlepeer-review

57 Citations (Scopus)


Dark fermentation is a technically feasible technology for achieving carbon dioxide-free hydrogen production. This review presents the current findings on continuous hydrogen production using dark fermentation. Several operational strategies and reactor configurations have been suggested. The formation of attached mixed-culture microorganisms is a typical prerequisite for achieving high production rate, hydrogen yield, and resilience. To date, fixed-bed reactors and dynamic membrane bioreactors yielded higher biohydrogen performance than other configurations. The symbiosis between H2-producing bacteria and biofilm-forming bacteria was essential to avoid washout and maintain the high loading rates and hydrogenic metabolic flux. Recent research has initiated a more in-depth comparison of microbial community changes during dark fermentation, primarily with computational science techniques based on 16S rRNA gene sequencing investigations. Future techno-economic analysis of dark fermentative biohydrogen production and perspectives on unraveling mitigation mechanisms induced by attached microorganisms in dark fermentation processes are further discussed.

Original languageEnglish
Article number124304
JournalBioresource technology
Publication statusPublished - 2021 Jan

Bibliographical note

Funding Information:
This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2019M3E6A1065160 and 2020R1I1A1A01061546 ). This research was also supported by the Yonsei University Research Fund of 2020 (2020-12-0021).

Publisher Copyright:
© 2020 Elsevier Ltd

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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