Staurosporine-induced Ca2+ mobilization in rat mandibular salivary acini

Dong Min Shin, Yeun Ju Kim, Syng Ill Lee, Jeong Taeg Seo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of the protein kinase C inhibitor, staurosporine, on intracellular Ca2+ homeostasis was investigated in rat mandibular salivary acinar cells loaded with fura-2. Fura-2 fluorescence was measured at 510 nm while the excitation wavelength was alternated between 340 nm and 380 nm. The ratio of fluorescence intensity (F340/380) was used as an index of [Ca2+]i. Stimulation of acinar cells with 10 μM carbachol (CCh) induced a rapid increase in F340/380 followed by a slow decrease to a sustained elevated level. Addition of 1 μM staurosporine in the presence of CCh caused a further increase in F340/380. In order to examine whether the staurosporine-induced increase in F340/380 could be attributed either to the Ca2+ entry pathway or to the mobilization of Ca2+ from intracellular Ca2+ stores, 1 μM staurosporine was added in the presence of CCh after Ca2+ had been omitted from the perfusate. Even in the absence of extracellular Ca2+, F340/380 still increased slowly from 0.75 ± 0.05 to 1.57 ± 0.24 after a delay ranging between 5 min and 10 min. However, the IP3-sensitive intracellular Ca2+ stores did not seem to play a major role in this phenomenon because staurosporine still increased F340/380 by 0.6 ± 0.10 after the complete depletion of IP3-sensitive Ca2+ stores by the exposure of cells to 1 μM thapsigargin, a microsomal Ca2+ ATPase inhibitor, in Ca2+-free conditions. These results suggest that staurosporine mobilizes Ca2+ from IP3-insensitive intracellular Ca2+ stores in rat mandibular salivary acinar cells.

Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalEuropean Journal of Morphology
Volume36
Issue number2 SUPPL.
Publication statusPublished - 1998 Dec 1

Fingerprint

Staurosporine
calcium
Acinar Cells
rats
Carbachol
carbachol
acinar cells
Fluorescence
Thapsigargin
Calcium-Transporting ATPases
Protein C Inhibitor
Fura-2
Protein Kinase Inhibitors
Protein Kinase C
staurosporine
Homeostasis
fluorescence
Ca2-transporting ATPase
protein kinase C
wavelengths

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Shin, Dong Min ; Kim, Yeun Ju ; Lee, Syng Ill ; Seo, Jeong Taeg. / Staurosporine-induced Ca2+ mobilization in rat mandibular salivary acini. In: European Journal of Morphology. 1998 ; Vol. 36, No. 2 SUPPL. pp. 161-164.
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abstract = "The effect of the protein kinase C inhibitor, staurosporine, on intracellular Ca2+ homeostasis was investigated in rat mandibular salivary acinar cells loaded with fura-2. Fura-2 fluorescence was measured at 510 nm while the excitation wavelength was alternated between 340 nm and 380 nm. The ratio of fluorescence intensity (F340/380) was used as an index of [Ca2+]i. Stimulation of acinar cells with 10 μM carbachol (CCh) induced a rapid increase in F340/380 followed by a slow decrease to a sustained elevated level. Addition of 1 μM staurosporine in the presence of CCh caused a further increase in F340/380. In order to examine whether the staurosporine-induced increase in F340/380 could be attributed either to the Ca2+ entry pathway or to the mobilization of Ca2+ from intracellular Ca2+ stores, 1 μM staurosporine was added in the presence of CCh after Ca2+ had been omitted from the perfusate. Even in the absence of extracellular Ca2+, F340/380 still increased slowly from 0.75 ± 0.05 to 1.57 ± 0.24 after a delay ranging between 5 min and 10 min. However, the IP3-sensitive intracellular Ca2+ stores did not seem to play a major role in this phenomenon because staurosporine still increased F340/380 by 0.6 ± 0.10 after the complete depletion of IP3-sensitive Ca2+ stores by the exposure of cells to 1 μM thapsigargin, a microsomal Ca2+ ATPase inhibitor, in Ca2+-free conditions. These results suggest that staurosporine mobilizes Ca2+ from IP3-insensitive intracellular Ca2+ stores in rat mandibular salivary acinar cells.",
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Shin, DM, Kim, YJ, Lee, SI & Seo, JT 1998, 'Staurosporine-induced Ca2+ mobilization in rat mandibular salivary acini', European Journal of Morphology, vol. 36, no. 2 SUPPL., pp. 161-164.

Staurosporine-induced Ca2+ mobilization in rat mandibular salivary acini. / Shin, Dong Min; Kim, Yeun Ju; Lee, Syng Ill; Seo, Jeong Taeg.

In: European Journal of Morphology, Vol. 36, No. 2 SUPPL., 01.12.1998, p. 161-164.

Research output: Contribution to journalArticle

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AU - Kim, Yeun Ju

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N2 - The effect of the protein kinase C inhibitor, staurosporine, on intracellular Ca2+ homeostasis was investigated in rat mandibular salivary acinar cells loaded with fura-2. Fura-2 fluorescence was measured at 510 nm while the excitation wavelength was alternated between 340 nm and 380 nm. The ratio of fluorescence intensity (F340/380) was used as an index of [Ca2+]i. Stimulation of acinar cells with 10 μM carbachol (CCh) induced a rapid increase in F340/380 followed by a slow decrease to a sustained elevated level. Addition of 1 μM staurosporine in the presence of CCh caused a further increase in F340/380. In order to examine whether the staurosporine-induced increase in F340/380 could be attributed either to the Ca2+ entry pathway or to the mobilization of Ca2+ from intracellular Ca2+ stores, 1 μM staurosporine was added in the presence of CCh after Ca2+ had been omitted from the perfusate. Even in the absence of extracellular Ca2+, F340/380 still increased slowly from 0.75 ± 0.05 to 1.57 ± 0.24 after a delay ranging between 5 min and 10 min. However, the IP3-sensitive intracellular Ca2+ stores did not seem to play a major role in this phenomenon because staurosporine still increased F340/380 by 0.6 ± 0.10 after the complete depletion of IP3-sensitive Ca2+ stores by the exposure of cells to 1 μM thapsigargin, a microsomal Ca2+ ATPase inhibitor, in Ca2+-free conditions. These results suggest that staurosporine mobilizes Ca2+ from IP3-insensitive intracellular Ca2+ stores in rat mandibular salivary acinar cells.

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