Staurosporine-inhibitable protein kinase activity associated with secretory granule membranes isolated from rat submandibular gland cells

Su Ryeon Seo, Yeun Ju Kim, Seok Jun Moon, Hiroshi Sugiya, DongMin Shin, Syng Ill Lee, Jeong Taeg Seo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Protein kinases, such as protein kinase C, have been shown to be associated with secretory granules and to regulate the event of exocytosis in various tissues including parotid salivary acinar cells. However, in submandibular acinar cells that play an important role in the secretion of proteins into the oral cavity, kinase activity on the granule membrane has not been explored. Therefore, in the present study, we isolated the secretory granules from rat submandibular acinar cells and investigated the localisation of protein kinases on the granule membrane. Initially, we isolated and purified secretory granules from rat submandibular acinar cells. Addition of [γ- 32 P] ATP to granule-membrane lysate phosphorylated the granule-membrane-associated 26, 32, 55 and 58 kDa proteins, sussestins the presence of endosenous kinase activity on the membrane. Moreover, the phosphorylation of 26 and 32 kDa proteins was inhibited by staurosporine and K252a, both non-specific protein kinase C inhibitors. However, the phosphorylation of 26 and 32 kDa proteins was not inhibited by other protein kinase C inhibitors, such as calphostin C, GF109203X and chelerythrine, indicatins that protein kinase C was not responsible for the phosphorylation. In addition, H-89, ML-9, KN-62 and genistein did not appear to inhibit this phosphorylation, indicating that protein kinase A, myosin light chain kinase (MLCK), Ca 2+ /calmodulin-dependent protein kinase II (CAMKII) and tyrosine kinase were not involved in the phosphorylation of 26 and 32 kDa proteins. Moreover, Ca 2+ had no effect on the kinase activity. Therefore, our results suggest that an unidentified, staurosporine-inhibitable protein kinase activity is associated with the secretory granule membrane of rat submandibular acinar cells.

Original languageEnglish
Pages (from-to)553-558
Number of pages6
JournalArchives of Oral Biology
Volume48
Issue number8
DOIs
Publication statusPublished - 2003 Aug 1

Fingerprint

Staurosporine
Submandibular Gland
Secretory Vesicles
Acinar Cells
Protein Kinases
Protein Kinase C
Phosphorylation
Membranes
KN 62
Phosphotransferases
Protein C Inhibitor
Protein Kinase Inhibitors
Proteins
Myosin-Light-Chain Kinase
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases
Genistein
Exocytosis
Cyclic AMP-Dependent Protein Kinases
Protein-Tyrosine Kinases

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Seo, Su Ryeon ; Kim, Yeun Ju ; Moon, Seok Jun ; Sugiya, Hiroshi ; Shin, DongMin ; Lee, Syng Ill ; Seo, Jeong Taeg. / Staurosporine-inhibitable protein kinase activity associated with secretory granule membranes isolated from rat submandibular gland cells. In: Archives of Oral Biology. 2003 ; Vol. 48, No. 8. pp. 553-558.
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Staurosporine-inhibitable protein kinase activity associated with secretory granule membranes isolated from rat submandibular gland cells. / Seo, Su Ryeon; Kim, Yeun Ju; Moon, Seok Jun; Sugiya, Hiroshi; Shin, DongMin; Lee, Syng Ill; Seo, Jeong Taeg.

In: Archives of Oral Biology, Vol. 48, No. 8, 01.08.2003, p. 553-558.

Research output: Contribution to journalArticle

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AU - Seo, Su Ryeon

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