Staurosporine mobilizes Ca 2+ from secretory granules by inhibiting protein kinase C in rat submandibular acinar cells

Y. J. Kim, J. M. An, DongMin Shin, S. I. Lee, H. Sugiya, J. T. Seo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Staurosporine was previously shown to mobilize Ca 2+ from the thapsigargin-insensitive Ca 2+ store in rat submandibular acinar cells. However, the nature of the store is not yet known. Therefore, in the present study, the staurosporine-releasable intracellular Ca 2+ store was characterized. Staurosporine increased the cytosolic Ca 2+ concentration ([Ca 2+ ] c ) after the inositol 1,4,5-trisphosphate (IP 3 )-sensitive Ca 2+ store was depleted. Ionomycin caused only small increases in [Ca 2+ ] c after the depletion of the IP 3 -sensitive Ca 2+ store, whereas ionomycin+monensin caused large increases. However, ionomycin+monensin did not increase [Ca 2+ ] c when added after [Ca 2+ ] c was increased by staurosporine, indicating that the acidic Ca 2+ store was the main source of Ca 2+ . The acidic Ca 2+ store appeared to be associated with secretory granules, since ionomycin+monensin-and staurosporine-induced [Ca 2+ ] c increases were significantly reduced when the acinar cells were degranulated. The effect of staurosporine on [Ca 2+ ] c was mimicked by other protein kinase C inhibitors. Therefore, we conclude that staurosporine mobilizes Ca 2+ from secretory granules, probably through the inhibition of protein kinase C in rat submandibular acinar cells.

Original languageEnglish
Pages (from-to)788-793
Number of pages6
JournalJournal of Dental Research
Volume81
Issue number11
Publication statusPublished - 2002 Nov 1

Fingerprint

Staurosporine
Acinar Cells
Secretory Vesicles
Protein Kinase C
Ionomycin
Monensin
Inositol 1,4,5-Trisphosphate
Thapsigargin
Protein C Inhibitor
Protein Kinase Inhibitors

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

@article{bce64e19e15747ef83689c399c718b0c,
title = "Staurosporine mobilizes Ca 2+ from secretory granules by inhibiting protein kinase C in rat submandibular acinar cells",
abstract = "Staurosporine was previously shown to mobilize Ca 2+ from the thapsigargin-insensitive Ca 2+ store in rat submandibular acinar cells. However, the nature of the store is not yet known. Therefore, in the present study, the staurosporine-releasable intracellular Ca 2+ store was characterized. Staurosporine increased the cytosolic Ca 2+ concentration ([Ca 2+ ] c ) after the inositol 1,4,5-trisphosphate (IP 3 )-sensitive Ca 2+ store was depleted. Ionomycin caused only small increases in [Ca 2+ ] c after the depletion of the IP 3 -sensitive Ca 2+ store, whereas ionomycin+monensin caused large increases. However, ionomycin+monensin did not increase [Ca 2+ ] c when added after [Ca 2+ ] c was increased by staurosporine, indicating that the acidic Ca 2+ store was the main source of Ca 2+ . The acidic Ca 2+ store appeared to be associated with secretory granules, since ionomycin+monensin-and staurosporine-induced [Ca 2+ ] c increases were significantly reduced when the acinar cells were degranulated. The effect of staurosporine on [Ca 2+ ] c was mimicked by other protein kinase C inhibitors. Therefore, we conclude that staurosporine mobilizes Ca 2+ from secretory granules, probably through the inhibition of protein kinase C in rat submandibular acinar cells.",
author = "Kim, {Y. J.} and An, {J. M.} and DongMin Shin and Lee, {S. I.} and H. Sugiya and Seo, {J. T.}",
year = "2002",
month = "11",
day = "1",
language = "English",
volume = "81",
pages = "788--793",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "11",

}

Staurosporine mobilizes Ca 2+ from secretory granules by inhibiting protein kinase C in rat submandibular acinar cells . / Kim, Y. J.; An, J. M.; Shin, DongMin; Lee, S. I.; Sugiya, H.; Seo, J. T.

In: Journal of Dental Research, Vol. 81, No. 11, 01.11.2002, p. 788-793.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Staurosporine mobilizes Ca 2+ from secretory granules by inhibiting protein kinase C in rat submandibular acinar cells

AU - Kim, Y. J.

AU - An, J. M.

AU - Shin, DongMin

AU - Lee, S. I.

AU - Sugiya, H.

AU - Seo, J. T.

PY - 2002/11/1

Y1 - 2002/11/1

N2 - Staurosporine was previously shown to mobilize Ca 2+ from the thapsigargin-insensitive Ca 2+ store in rat submandibular acinar cells. However, the nature of the store is not yet known. Therefore, in the present study, the staurosporine-releasable intracellular Ca 2+ store was characterized. Staurosporine increased the cytosolic Ca 2+ concentration ([Ca 2+ ] c ) after the inositol 1,4,5-trisphosphate (IP 3 )-sensitive Ca 2+ store was depleted. Ionomycin caused only small increases in [Ca 2+ ] c after the depletion of the IP 3 -sensitive Ca 2+ store, whereas ionomycin+monensin caused large increases. However, ionomycin+monensin did not increase [Ca 2+ ] c when added after [Ca 2+ ] c was increased by staurosporine, indicating that the acidic Ca 2+ store was the main source of Ca 2+ . The acidic Ca 2+ store appeared to be associated with secretory granules, since ionomycin+monensin-and staurosporine-induced [Ca 2+ ] c increases were significantly reduced when the acinar cells were degranulated. The effect of staurosporine on [Ca 2+ ] c was mimicked by other protein kinase C inhibitors. Therefore, we conclude that staurosporine mobilizes Ca 2+ from secretory granules, probably through the inhibition of protein kinase C in rat submandibular acinar cells.

AB - Staurosporine was previously shown to mobilize Ca 2+ from the thapsigargin-insensitive Ca 2+ store in rat submandibular acinar cells. However, the nature of the store is not yet known. Therefore, in the present study, the staurosporine-releasable intracellular Ca 2+ store was characterized. Staurosporine increased the cytosolic Ca 2+ concentration ([Ca 2+ ] c ) after the inositol 1,4,5-trisphosphate (IP 3 )-sensitive Ca 2+ store was depleted. Ionomycin caused only small increases in [Ca 2+ ] c after the depletion of the IP 3 -sensitive Ca 2+ store, whereas ionomycin+monensin caused large increases. However, ionomycin+monensin did not increase [Ca 2+ ] c when added after [Ca 2+ ] c was increased by staurosporine, indicating that the acidic Ca 2+ store was the main source of Ca 2+ . The acidic Ca 2+ store appeared to be associated with secretory granules, since ionomycin+monensin-and staurosporine-induced [Ca 2+ ] c increases were significantly reduced when the acinar cells were degranulated. The effect of staurosporine on [Ca 2+ ] c was mimicked by other protein kinase C inhibitors. Therefore, we conclude that staurosporine mobilizes Ca 2+ from secretory granules, probably through the inhibition of protein kinase C in rat submandibular acinar cells.

UR - http://www.scopus.com/inward/record.url?scp=0036828168&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036828168&partnerID=8YFLogxK

M3 - Article

VL - 81

SP - 788

EP - 793

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 11

ER -