Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images

Jihun Kang; Eun Hye Kang; Young Shik Yun; Seungmuk Ji; In Sik Yun; Jong Souk Yeo

doi:10.1186/s42649-020-00046-3

Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images

Jihun Kang, Eun Hye Kang, Young Shik Yun, Seungmuk Ji, In Sik Yun, Jong Souk Yeo

School of Integrated Technology

Research output: Contribution to journal › Article › peer-review

Abstract

The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.

Original language	English
Article number	26
Journal	Applied Microscopy
Volume	50
Issue number	1
DOIs	https://doi.org/10.1186/s42649-020-00046-3
Publication status	Published - 2020 Dec

Bibliographical note

Funding Information:
This research was supported by Research Program through the National Institute of Ecology (NIE-Basic Research-2020-18) and also supported by National Research Foundation (NRF) grant funded by the Ministry of Education, under “Basic Science Research Program” (NRF-2017R1D1A1B04033182).

Publisher Copyright:
© 2020, The Author(s).

All Science Journal Classification (ASJC) codes

Microbiology
Applied Microbiology and Biotechnology

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10.1186/s42649-020-00046-3

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title = "Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images",

abstract = "The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.",

author = "Jihun Kang and Kang, {Eun Hye} and Yun, {Young Shik} and Seungmuk Ji and Yun, {In Sik} and Yeo, {Jong Souk}",

note = "Funding Information: This research was supported by Research Program through the National Institute of Ecology (NIE-Basic Research-2020-18) and also supported by National Research Foundation (NRF) grant funded by the Ministry of Education, under “Basic Science Research Program” (NRF-2017R1D1A1B04033182). Publisher Copyright: {\textcopyright} 2020, The Author(s).",

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doi = "10.1186/s42649-020-00046-3",

language = "English",

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AU - Kang, Jihun

AU - Kang, Eun Hye

AU - Yun, Young Shik

AU - Ji, Seungmuk

AU - Yun, In Sik

AU - Yeo, Jong Souk

N1 - Funding Information: This research was supported by Research Program through the National Institute of Ecology (NIE-Basic Research-2020-18) and also supported by National Research Foundation (NRF) grant funded by the Ministry of Education, under “Basic Science Research Program” (NRF-2017R1D1A1B04033182). Publisher Copyright: © 2020, The Author(s).

PY - 2020/12

Y1 - 2020/12

N2 - The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.

AB - The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.

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Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images

Abstract

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