Stem cell passage affects directional migration of stem cells in electrotaxis

Seung Hee Hong, Mi Hee Lee, Min Ah Koo, Gyeung Mi Seon, Ye Jin Park, Dohyun Kim, Jong Chul Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Stem cells can differentiate into various body tissues and organs and thus are considered as promising tools for cell therapy and tissue engineering. Early passage stem cells have high differentiation ability compared to late passage stem cells. Thus, it is important to use early passage stem cells in cell therapy. Here, we investigated whether cell migration could be used to compare young and senescent cells. We used ‘electrotaxis’ where cells under electric treatment move towards the anode or cathode. Without an electric stimulus, stem cells moved randomly. However, under a direct electric current, the cells moved with directionality. Under stimulation with a direct electric current, early passage stem cells moved towards the anode; when the cells became senescent with increasing passages, the percentage of cells migrating to the anode decreased. These results suggest that the behavior of stem cells under the influence of a direct electric current is also related to their passage number. Therefore, electrotaxis migration analysis can be used to distinguish between young cell and senescent cells.

Original languageEnglish
Article number101475
JournalStem Cell Research
Volume38
DOIs
Publication statusPublished - 2019 Jul

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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