Sterilization of sealed PVDF pouches containing decellularized scaffold by electrical stimulation

Mi Hee Lee, Ha Kyeong Jeong, Min Ah Koo, Gyeung Mi Seon, Seung Hee Hong, Ye Jin Park, Jong Chul Park

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


A terminal sterilization process for tissue engineering products, such as allografts and biomaterials is necessary to ensure complete removal of pathogenic microorganisms such as the bacteria, fungi, and viruses. However, it can be difficult to sterilize allografts and artificial tissue models packaged in wet conditions without deformation. In this study, we investigated the sterilization effects of electrical stimulation (ES) and assessed its suitability by evaluating sterility assurance levels in pouches at a constant current. Stability of polyvinylidene fluoride pouches was determined by a sterility test performed after exposure to five microorganisms (Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans) for 5 days; the sterility test was also performed with decellularized human dermal tissues inoculated with the five microorganisms. Sterilization using ES inactivated microorganisms both inside and outside of sealed pouches and caused no damage to the packaged tissue. Our results support the development of a novel system that involves ES sterilization for packaging of implantable biomaterials and human derived materials.

Original languageEnglish
Article number2100156
JournalBiotechnology Journal
Issue number11
Publication statusPublished - 2021 Nov

Bibliographical note

Funding Information:
This research was supported by a National Research Foundation (NRF) grant funded by the Korean government (MSIT) (No. 2017M3A9B3063638, No. 2019R1A2C2005256). The authors would like to thank L&C Bio for friendly donating the decellularized human dermal tissue.

Publisher Copyright:
© 2021 Wiley-VCH GmbH

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Molecular Medicine


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