Steroidogenic factor 1 regulates expression of the cannabinoid receptor 1 in the ventromedial hypothalamic nucleus

Ki Woo Kim, Young Hwan Jo, Liping Zhao, Nancy R. Stallings, Streamson C. Chua, Keith L. Parker

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Abstract

The nuclear receptor steroidogenic factor 1 (SF-1) plays essential roles in the development and function of the ventromedial hypothalamic nucleus (VMH). Considerable evidence links the VMH and SF-1 with the regulation of energy homeostasis. Here, we demonstrate that SF-1 colocalizes in VMH neurons with the cannabinoid receptor 1 (CB1R) and that a specific CB1R agonist modulates electrical activity of SF-1 neurons in hypothalamic slice preparations. We further show that SF-1 directly regulates CB1R geneexpression via a SF-1-responsive element at -101 in its 5-flanking region. Finally, we show that knockout mice with selective inactivation of SF-1 in the brain have decreased expression of CB1R in the region of the VMH and exhibit a blunted response to systemi-cally administered CB1R agonists. These studies suggest that SF-1 directly regulates the expression of CB1R, which has been implicated in the regulation of energy homeostasis and anxiety-like behavior.

Original languageEnglish
Pages (from-to)1950-1961
Number of pages12
JournalMolecular Endocrinology
Volume22
Issue number8
DOIs
Publication statusPublished - 2008 Aug 1

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Endocrinology

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