Sticky "delivering-from" strategies using viral vectors for efficient human neural stem cell infection by bioinspired catecholamines

Eunmi Kim, Slgirim Lee, Seonki Hong, Gyuhyung Jin, Minhee Kim, Kook In Park, Haeshin Lee, Jae-Hyung Jang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Controlled release of biosuprastructures, such as viruses, from surfaces has been a challenging task in providing efficient ex vivo gene delivery. Conventional controlled viral release approaches have demonstrated low viral immobilization and burst release, inhibiting delivery efficiency. Here, a highly powerful substrate-mediated viral delivery system was designed by combining two key components that have demonstrated great potential in the fields of gene therapy and surface chemistry, respectively: adeno-associated viral (AAV) vectors and adhesive catecholamine surfaces. The introduction of a nanoscale thin coating of catecholamines, poly(norepinephrine) (pNE) or poly(dopamine) (pDA) to provide AAV adhesion followed by human neural stem cell (hNSC) culture on sticky solid surfaces exhibited unprecedented results: approximately 90% loading vs 25% (AAV-bare surface), no burst release, sustained release at constant rates, approximately 70% infection vs 20% (AAV-bare surface), and rapid internalization. Importantly, the sticky catecholamine-mediated AAV delivery system successfully induced a physiological response from hNSCs, cellular proliferation by a single-shot of AAV encoding fibroblast growth factor-2 (FGF-2), which is typically achieved by multiple treatments with expensive FGF-2 proteins. By combining the adhesive material-independent surface functionalization characters of pNE and pDA, this new sticky "delivering-from" gene delivery platform will make a significant contribution to numerous fields, including tissue engineering, gene therapy, and stem cell therapy.

Original languageEnglish
Pages (from-to)8288-8294
Number of pages7
JournalACS Applied Materials and Interfaces
Volume6
Issue number11
DOIs
Publication statusPublished - 2014 Jun 11

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Stem cells
Catecholamines
Norepinephrine
Gene therapy
Fibroblast Growth Factor 2
Fibroblasts
Adhesives
Genes
Surface chemistry
Viruses
Tissue engineering
Cell culture
Adhesion
Proteins
Coatings
Substrates

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Kim, Eunmi ; Lee, Slgirim ; Hong, Seonki ; Jin, Gyuhyung ; Kim, Minhee ; Park, Kook In ; Lee, Haeshin ; Jang, Jae-Hyung. / Sticky "delivering-from" strategies using viral vectors for efficient human neural stem cell infection by bioinspired catecholamines. In: ACS Applied Materials and Interfaces. 2014 ; Vol. 6, No. 11. pp. 8288-8294.
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Sticky "delivering-from" strategies using viral vectors for efficient human neural stem cell infection by bioinspired catecholamines. / Kim, Eunmi; Lee, Slgirim; Hong, Seonki; Jin, Gyuhyung; Kim, Minhee; Park, Kook In; Lee, Haeshin; Jang, Jae-Hyung.

In: ACS Applied Materials and Interfaces, Vol. 6, No. 11, 11.06.2014, p. 8288-8294.

Research output: Contribution to journalArticle

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