Sticky "delivering-from" strategies using viral vectors for efficient human neural stem cell infection by bioinspired catecholamines

Eunmi Kim, Slgirim Lee, Seonki Hong, Gyuhyung Jin, Minhee Kim, Kook In Park, Haeshin Lee, Jae Hyung Jang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Controlled release of biosuprastructures, such as viruses, from surfaces has been a challenging task in providing efficient ex vivo gene delivery. Conventional controlled viral release approaches have demonstrated low viral immobilization and burst release, inhibiting delivery efficiency. Here, a highly powerful substrate-mediated viral delivery system was designed by combining two key components that have demonstrated great potential in the fields of gene therapy and surface chemistry, respectively: adeno-associated viral (AAV) vectors and adhesive catecholamine surfaces. The introduction of a nanoscale thin coating of catecholamines, poly(norepinephrine) (pNE) or poly(dopamine) (pDA) to provide AAV adhesion followed by human neural stem cell (hNSC) culture on sticky solid surfaces exhibited unprecedented results: approximately 90% loading vs 25% (AAV-bare surface), no burst release, sustained release at constant rates, approximately 70% infection vs 20% (AAV-bare surface), and rapid internalization. Importantly, the sticky catecholamine-mediated AAV delivery system successfully induced a physiological response from hNSCs, cellular proliferation by a single-shot of AAV encoding fibroblast growth factor-2 (FGF-2), which is typically achieved by multiple treatments with expensive FGF-2 proteins. By combining the adhesive material-independent surface functionalization characters of pNE and pDA, this new sticky "delivering-from" gene delivery platform will make a significant contribution to numerous fields, including tissue engineering, gene therapy, and stem cell therapy.

Original languageEnglish
Pages (from-to)8288-8294
Number of pages7
JournalACS Applied Materials and Interfaces
Volume6
Issue number11
DOIs
Publication statusPublished - 2014 Jun 11

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

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