Stimulating effect of graphene oxide on myogenesis of C2C12 myoblasts on RGD peptide-decorated PLGA nanofiber matrices

Yong Cheol Shin, Jong Ho Lee, Min Jeong Kim, Suck Won Hong, Bongju Kim, Jung Keun Hyun, Yu Suk Choi, Jong Chul Park, Dong Wook Han

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: In the field of biomedical engineering, many studies have focused on the possible applications of graphene and related nanomaterials due to their potential for use as scaffolds, coating materials and delivery carriers. On the other hand, electrospun nanofiber matrices composed of diverse biocompatible polymers have attracted tremendous attention for tissue engineering and regenerative medicine. However, their combination is intriguing and still challenging. Results: In the present study, we fabricated nanofiber matrices composed of M13 bacteriophage with RGD peptide displayed on its surface (RGD-M13 phage) and poly(lactic-co-glycolic acid, PLGA) and characterized their physicochemical properties. In addition, the effect of graphene oxide (GO) on the cellular behaviors of C2C12 myoblasts, which were cultured on PLGA decorated with RGD-M13 phage (RGD/PLGA) nanofiber matrices, was investigated. Our results revealed that the RGD/PLGA nanofiber matrices have suitable physicochemical properties as a tissue engineering scaffold and the growth of C2C12 myoblasts were significantly enhanced on the matrices. Moreover, the myogenic differentiation of C2C12 myoblasts was substantially stimulated when they were cultured on the RGD/PLGA matrices in the presence of GO. Conclusion: In conclusion, these findings propose that the combination of RGD/PLGA nanofiber matrices and GO can be used as a promising strategy for skeletal tissue engineering and regeneration.

Original languageEnglish
Article number22
JournalJournal of Biological Engineering
Volume9
Issue number1
DOIs
Publication statusPublished - 2015 Nov 25

Fingerprint

Nanofibers
Graphite
Muscle Development
Myoblasts
Oxides
Graphene
Peptides
Bacteriophages
Bacteriophage M13
Tissue engineering
Tissue Engineering
Tissue regeneration
Biomedical engineering
Scaffolds (biology)
Biomedical Engineering
Nanostructured materials
Tissue Scaffolds
Scaffolds
Regenerative Medicine
Nanostructures

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Biomedical Engineering
  • Molecular Biology
  • Cell Biology

Cite this

Shin, Yong Cheol ; Lee, Jong Ho ; Kim, Min Jeong ; Hong, Suck Won ; Kim, Bongju ; Hyun, Jung Keun ; Choi, Yu Suk ; Park, Jong Chul ; Han, Dong Wook. / Stimulating effect of graphene oxide on myogenesis of C2C12 myoblasts on RGD peptide-decorated PLGA nanofiber matrices. In: Journal of Biological Engineering. 2015 ; Vol. 9, No. 1.
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Stimulating effect of graphene oxide on myogenesis of C2C12 myoblasts on RGD peptide-decorated PLGA nanofiber matrices. / Shin, Yong Cheol; Lee, Jong Ho; Kim, Min Jeong; Hong, Suck Won; Kim, Bongju; Hyun, Jung Keun; Choi, Yu Suk; Park, Jong Chul; Han, Dong Wook.

In: Journal of Biological Engineering, Vol. 9, No. 1, 22, 25.11.2015.

Research output: Contribution to journalArticle

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AU - Shin, Yong Cheol

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AU - Hong, Suck Won

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AU - Park, Jong Chul

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