Stimulation of epidermal calcium gradient loss increases the expression of hyaluronan and CD44 in mouse skin

S. E. Lee, J. E. Jun, Eung Ho Choi, S. K. Ahn, S. H. Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background. Hyaluronan (HA), a major extracellular matrix component in epidermis, has been found to accumulate in the epidermis after disruption of the epidermal barrier; however, the precise mechanisms underlying this process are not yet clear. Alterations in the epidermal calcium gradient are an important signal for permeability-barrier homeostasis. Thus, we hypothesized that epidermal calcium-ions might regulate HA expression. Aim. To investigate whether changes in the epidermal calcium gradient and subsequent induction of cytokines regulate HA, HA synthase (HAS) and HA receptor (CD44) expression in mouse epidermis, and to clarify the mechanisms of HA induction. Methods. Sonophoresis of 1.5 mmol/L Ca2+-containing gel or Ca2+-free gel was performed to manipulate the epidermal Ca2+ content without disrupting the permeability barrier. We also manipulated the Ca2+ gradient by tape-stripping with or without 2 h immersion in 1.2 mmol/L Ca 2+-containing solutions. Next we inhibited cytokine activity using tumour necrosis factor (TNF)-α or interleukin (IL)-1 inhibitors before sonophoresis. Six hours after each treatment, the expression of HA, HAS and CD44 were analysed using reverse transcription PCR and immunohistochemical stains. Results. Sonophoresis of Ca2+-free gel significantly increased HA, HAS3 and CD44 expression in epidermis and in tape-stripped skin. However, the inhibition of Ca2+ decrease in the upper epidermis by sonophoresis of Ca2+-containing gel or immersion of barrier-disrupted skin into a Ca2+-containing solution attenuated these inductions. Specific inhibitors of TNF-α and IL-1 specific inhibitors also abolished the sonophoresis-induced expression of HA, HAS3 and CD44. Conclusions. These results suggest that modulations in epidermal calcium regulate HA and CD44 expression directly or via induction of cytokines.

Original languageEnglish
Pages (from-to)650-657
Number of pages8
JournalClinical and Experimental Dermatology
Volume35
Issue number6
DOIs
Publication statusPublished - 2010 Aug 1

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Hyaluronic Acid
Epidermis
Calcium
Skin
Gels
Immersion
Cytokines
Interleukin-1
Permeability
Surgical Tape
Tumor Necrosis Factor-alpha
CD44 Antigens
Reverse Transcription
Extracellular Matrix
Homeostasis
Coloring Agents
Ions
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

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title = "Stimulation of epidermal calcium gradient loss increases the expression of hyaluronan and CD44 in mouse skin",
abstract = "Background. Hyaluronan (HA), a major extracellular matrix component in epidermis, has been found to accumulate in the epidermis after disruption of the epidermal barrier; however, the precise mechanisms underlying this process are not yet clear. Alterations in the epidermal calcium gradient are an important signal for permeability-barrier homeostasis. Thus, we hypothesized that epidermal calcium-ions might regulate HA expression. Aim. To investigate whether changes in the epidermal calcium gradient and subsequent induction of cytokines regulate HA, HA synthase (HAS) and HA receptor (CD44) expression in mouse epidermis, and to clarify the mechanisms of HA induction. Methods. Sonophoresis of 1.5 mmol/L Ca2+-containing gel or Ca2+-free gel was performed to manipulate the epidermal Ca2+ content without disrupting the permeability barrier. We also manipulated the Ca2+ gradient by tape-stripping with or without 2 h immersion in 1.2 mmol/L Ca 2+-containing solutions. Next we inhibited cytokine activity using tumour necrosis factor (TNF)-α or interleukin (IL)-1 inhibitors before sonophoresis. Six hours after each treatment, the expression of HA, HAS and CD44 were analysed using reverse transcription PCR and immunohistochemical stains. Results. Sonophoresis of Ca2+-free gel significantly increased HA, HAS3 and CD44 expression in epidermis and in tape-stripped skin. However, the inhibition of Ca2+ decrease in the upper epidermis by sonophoresis of Ca2+-containing gel or immersion of barrier-disrupted skin into a Ca2+-containing solution attenuated these inductions. Specific inhibitors of TNF-α and IL-1 specific inhibitors also abolished the sonophoresis-induced expression of HA, HAS3 and CD44. Conclusions. These results suggest that modulations in epidermal calcium regulate HA and CD44 expression directly or via induction of cytokines.",
author = "Lee, {S. E.} and Jun, {J. E.} and Choi, {Eung Ho} and Ahn, {S. K.} and Lee, {S. H.}",
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Stimulation of epidermal calcium gradient loss increases the expression of hyaluronan and CD44 in mouse skin. / Lee, S. E.; Jun, J. E.; Choi, Eung Ho; Ahn, S. K.; Lee, S. H.

In: Clinical and Experimental Dermatology, Vol. 35, No. 6, 01.08.2010, p. 650-657.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stimulation of epidermal calcium gradient loss increases the expression of hyaluronan and CD44 in mouse skin

AU - Lee, S. E.

AU - Jun, J. E.

AU - Choi, Eung Ho

AU - Ahn, S. K.

AU - Lee, S. H.

PY - 2010/8/1

Y1 - 2010/8/1

N2 - Background. Hyaluronan (HA), a major extracellular matrix component in epidermis, has been found to accumulate in the epidermis after disruption of the epidermal barrier; however, the precise mechanisms underlying this process are not yet clear. Alterations in the epidermal calcium gradient are an important signal for permeability-barrier homeostasis. Thus, we hypothesized that epidermal calcium-ions might regulate HA expression. Aim. To investigate whether changes in the epidermal calcium gradient and subsequent induction of cytokines regulate HA, HA synthase (HAS) and HA receptor (CD44) expression in mouse epidermis, and to clarify the mechanisms of HA induction. Methods. Sonophoresis of 1.5 mmol/L Ca2+-containing gel or Ca2+-free gel was performed to manipulate the epidermal Ca2+ content without disrupting the permeability barrier. We also manipulated the Ca2+ gradient by tape-stripping with or without 2 h immersion in 1.2 mmol/L Ca 2+-containing solutions. Next we inhibited cytokine activity using tumour necrosis factor (TNF)-α or interleukin (IL)-1 inhibitors before sonophoresis. Six hours after each treatment, the expression of HA, HAS and CD44 were analysed using reverse transcription PCR and immunohistochemical stains. Results. Sonophoresis of Ca2+-free gel significantly increased HA, HAS3 and CD44 expression in epidermis and in tape-stripped skin. However, the inhibition of Ca2+ decrease in the upper epidermis by sonophoresis of Ca2+-containing gel or immersion of barrier-disrupted skin into a Ca2+-containing solution attenuated these inductions. Specific inhibitors of TNF-α and IL-1 specific inhibitors also abolished the sonophoresis-induced expression of HA, HAS3 and CD44. Conclusions. These results suggest that modulations in epidermal calcium regulate HA and CD44 expression directly or via induction of cytokines.

AB - Background. Hyaluronan (HA), a major extracellular matrix component in epidermis, has been found to accumulate in the epidermis after disruption of the epidermal barrier; however, the precise mechanisms underlying this process are not yet clear. Alterations in the epidermal calcium gradient are an important signal for permeability-barrier homeostasis. Thus, we hypothesized that epidermal calcium-ions might regulate HA expression. Aim. To investigate whether changes in the epidermal calcium gradient and subsequent induction of cytokines regulate HA, HA synthase (HAS) and HA receptor (CD44) expression in mouse epidermis, and to clarify the mechanisms of HA induction. Methods. Sonophoresis of 1.5 mmol/L Ca2+-containing gel or Ca2+-free gel was performed to manipulate the epidermal Ca2+ content without disrupting the permeability barrier. We also manipulated the Ca2+ gradient by tape-stripping with or without 2 h immersion in 1.2 mmol/L Ca 2+-containing solutions. Next we inhibited cytokine activity using tumour necrosis factor (TNF)-α or interleukin (IL)-1 inhibitors before sonophoresis. Six hours after each treatment, the expression of HA, HAS and CD44 were analysed using reverse transcription PCR and immunohistochemical stains. Results. Sonophoresis of Ca2+-free gel significantly increased HA, HAS3 and CD44 expression in epidermis and in tape-stripped skin. However, the inhibition of Ca2+ decrease in the upper epidermis by sonophoresis of Ca2+-containing gel or immersion of barrier-disrupted skin into a Ca2+-containing solution attenuated these inductions. Specific inhibitors of TNF-α and IL-1 specific inhibitors also abolished the sonophoresis-induced expression of HA, HAS3 and CD44. Conclusions. These results suggest that modulations in epidermal calcium regulate HA and CD44 expression directly or via induction of cytokines.

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