Stochastic electrotransport selectively enhances the transport of highly electromobile molecules

Sung Yon Kim, Jae Hun Cho, Evan Murray, Naveed Bakh, Heejin Choi, Kimberly Ohn, Luzdary Ruelas, Austin Hubbert, Meg McCue, Sara L. Vassallo, Philipp J. Keller, Kwanghun Chung

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Nondestructive chemical processing of porous samples such as fixed biological tissues typically relies on molecular diffusion. Diffusion into a porous structure is a slow process that significantly delays completion of chemical processing. Here, we present a novel electrokinetic method termed stochastic electrotransport for rapid nondestructive processing of porous samples. This method uses a rotational electric field to selectively disperse highly electromobile molecules throughout a porous sample without displacing the low-electromobility molecules that constitute the sample. Using computational models, we show that stochastic electrotransport can rapidly disperse electromobile molecules in a porous medium. We apply this method to completely clear mouse organs within 1-3 days and to stain them with nuclear dyes, proteins, and antibodies within 1 day. Our results demonstrate the potential of stochastic electrotransport to process large and dense tissue samples that were previously infeasible in time when relying on diffusion.

Original languageEnglish
Pages (from-to)E6274-E6283
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number46
DOIs
Publication statusPublished - 2015 Nov 17

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Coloring Agents
Nuclear Proteins
Antibodies

All Science Journal Classification (ASJC) codes

  • General

Cite this

Kim, Sung Yon ; Cho, Jae Hun ; Murray, Evan ; Bakh, Naveed ; Choi, Heejin ; Ohn, Kimberly ; Ruelas, Luzdary ; Hubbert, Austin ; McCue, Meg ; Vassallo, Sara L. ; Keller, Philipp J. ; Chung, Kwanghun. / Stochastic electrotransport selectively enhances the transport of highly electromobile molecules. In: Proceedings of the National Academy of Sciences of the United States of America. 2015 ; Vol. 112, No. 46. pp. E6274-E6283.
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Kim, SY, Cho, JH, Murray, E, Bakh, N, Choi, H, Ohn, K, Ruelas, L, Hubbert, A, McCue, M, Vassallo, SL, Keller, PJ & Chung, K 2015, 'Stochastic electrotransport selectively enhances the transport of highly electromobile molecules', Proceedings of the National Academy of Sciences of the United States of America, vol. 112, no. 46, pp. E6274-E6283. https://doi.org/10.1073/pnas.1510133112

Stochastic electrotransport selectively enhances the transport of highly electromobile molecules. / Kim, Sung Yon; Cho, Jae Hun; Murray, Evan; Bakh, Naveed; Choi, Heejin; Ohn, Kimberly; Ruelas, Luzdary; Hubbert, Austin; McCue, Meg; Vassallo, Sara L.; Keller, Philipp J.; Chung, Kwanghun.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 46, 17.11.2015, p. E6274-E6283.

Research output: Contribution to journalArticle

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AU - Ruelas, Luzdary

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