Stochastic optical active rheology

Hyungsuk Lee, Yongdae Shin, Sun Taek Kim, Ellis L. Reinherz, Matthew J. Lang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We demonstrate a stochastic based method for performing active rheology using optical tweezers. By monitoring the displacement of an embedded particle in response to stochastic optical forces, a rapid estimate of the frequency dependent shear moduli of a sample is achieved in the range of 10 -1-10 3 Hz. We utilize the method to probe linear viscoelastic properties of hydrogels at varied cross-linker concentrations. Combined with fluorescence imaging, our method demonstrates non-linear changes of bond strength between T cell receptors and an antigenic peptide due to force-induced cell activation.

Original languageEnglish
Article number031902
JournalApplied Physics Letters
Volume101
Issue number3
DOIs
Publication statusPublished - 2012 Jul 16

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rheology
peptides
activation
shear
fluorescence
probes
estimates
cells

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

Cite this

Lee, H., Shin, Y., Kim, S. T., Reinherz, E. L., & Lang, M. J. (2012). Stochastic optical active rheology. Applied Physics Letters, 101(3), [031902]. https://doi.org/10.1063/1.4737159
Lee, Hyungsuk ; Shin, Yongdae ; Kim, Sun Taek ; Reinherz, Ellis L. ; Lang, Matthew J. / Stochastic optical active rheology. In: Applied Physics Letters. 2012 ; Vol. 101, No. 3.
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Lee, H, Shin, Y, Kim, ST, Reinherz, EL & Lang, MJ 2012, 'Stochastic optical active rheology', Applied Physics Letters, vol. 101, no. 3, 031902. https://doi.org/10.1063/1.4737159

Stochastic optical active rheology. / Lee, Hyungsuk; Shin, Yongdae; Kim, Sun Taek; Reinherz, Ellis L.; Lang, Matthew J.

In: Applied Physics Letters, Vol. 101, No. 3, 031902, 16.07.2012.

Research output: Contribution to journalArticle

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Lee H, Shin Y, Kim ST, Reinherz EL, Lang MJ. Stochastic optical active rheology. Applied Physics Letters. 2012 Jul 16;101(3). 031902. https://doi.org/10.1063/1.4737159