Stochastic optical active rheology

Hyungsuk Lee, Yongdae Shin, Sun Taek Kim, Ellis L. Reinherz, Matthew J. Lang

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Abstract

We demonstrate a stochastic based method for performing active rheology using optical tweezers. By monitoring the displacement of an embedded particle in response to stochastic optical forces, a rapid estimate of the frequency dependent shear moduli of a sample is achieved in the range of 10 -1-10 3 Hz. We utilize the method to probe linear viscoelastic properties of hydrogels at varied cross-linker concentrations. Combined with fluorescence imaging, our method demonstrates non-linear changes of bond strength between T cell receptors and an antigenic peptide due to force-induced cell activation.

Original languageEnglish
Article number031902
JournalApplied Physics Letters
Volume101
Issue number3
DOIs
Publication statusPublished - 2012 Jul 16

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All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

Cite this

Lee, H., Shin, Y., Kim, S. T., Reinherz, E. L., & Lang, M. J. (2012). Stochastic optical active rheology. Applied Physics Letters, 101(3), [031902]. https://doi.org/10.1063/1.4737159