Abstract
The frit inlet asymmetrical flow field-flow fractionation is applied to the separation of proteins by using a stopless flow injection procedure. By utilizing a small permeable frit near the injection point in an asymmetrical flow FFF channel, sample materials injected to the flow streams can be hydrodynamically relaxed by the compressing action of high speed frit flow and the focusing/relaxation procedure can be bypassed. The separation efficiency of the frit inlet asymmetrical flow FFF channel is demonstrated with few protein standards by examining the influence of the ratio of injection flow rate to frit flow rate on the band broadening during relaxation.
| Original language | English |
|---|---|
| Pages (from-to) | 2803-2814 |
| Number of pages | 12 |
| Journal | Journal of Liquid Chromatography and Related Technologies |
| Volume | 20 |
| Issue number | 16-17 |
| DOIs | |
| Publication status | Published - 1997 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Pharmaceutical Science
- Clinical Biochemistry
Cite this
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Stopless separation of proteins by frit-inlet asymmetrical flow field-flow fractionation. / Moon, Myeong Hee; Kwon, Hansun; Park, Ilyong.
In: Journal of Liquid Chromatography and Related Technologies, Vol. 20, No. 16-17, 01.01.1997, p. 2803-2814.Research output: Contribution to journal › Article
TY - JOUR
T1 - Stopless separation of proteins by frit-inlet asymmetrical flow field-flow fractionation
AU - Moon, Myeong Hee
AU - Kwon, Hansun
AU - Park, Ilyong
PY - 1997/1/1
Y1 - 1997/1/1
N2 - The frit inlet asymmetrical flow field-flow fractionation is applied to the separation of proteins by using a stopless flow injection procedure. By utilizing a small permeable frit near the injection point in an asymmetrical flow FFF channel, sample materials injected to the flow streams can be hydrodynamically relaxed by the compressing action of high speed frit flow and the focusing/relaxation procedure can be bypassed. The separation efficiency of the frit inlet asymmetrical flow FFF channel is demonstrated with few protein standards by examining the influence of the ratio of injection flow rate to frit flow rate on the band broadening during relaxation.
AB - The frit inlet asymmetrical flow field-flow fractionation is applied to the separation of proteins by using a stopless flow injection procedure. By utilizing a small permeable frit near the injection point in an asymmetrical flow FFF channel, sample materials injected to the flow streams can be hydrodynamically relaxed by the compressing action of high speed frit flow and the focusing/relaxation procedure can be bypassed. The separation efficiency of the frit inlet asymmetrical flow FFF channel is demonstrated with few protein standards by examining the influence of the ratio of injection flow rate to frit flow rate on the band broadening during relaxation.
UR - http://www.scopus.com/inward/record.url?scp=0030827792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030827792&partnerID=8YFLogxK
U2 - 10.1080/10826079708005593
DO - 10.1080/10826079708005593
M3 - Article
AN - SCOPUS:0030827792
VL - 20
SP - 2803
EP - 2814
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
SN - 1082-6076
IS - 16-17
ER -