Stopless separation of proteins by frit-inlet asymmetrical flow field-flow fractionation

Myeong Hee Moon; Hansun Kwon; Ilyong Park

doi:10.1080/10826079708005593

Stopless separation of proteins by frit-inlet asymmetrical flow field-flow fractionation

Myeong Hee Moon, Hansun Kwon, Ilyong Park

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The frit inlet asymmetrical flow field-flow fractionation is applied to the separation of proteins by using a stopless flow injection procedure. By utilizing a small permeable frit near the injection point in an asymmetrical flow FFF channel, sample materials injected to the flow streams can be hydrodynamically relaxed by the compressing action of high speed frit flow and the focusing/relaxation procedure can be bypassed. The separation efficiency of the frit inlet asymmetrical flow FFF channel is demonstrated with few protein standards by examining the influence of the ratio of injection flow rate to frit flow rate on the band broadening during relaxation.

Original language	English
Pages (from-to)	2803-2814
Number of pages	12
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	20
Issue number	16-17
DOIs	https://doi.org/10.1080/10826079708005593
Publication status	Published - 1997

Bibliographical note

Funding Information:
This study was supported by KOSEF Fund 960501-7.

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Pharmaceutical Science
Clinical Biochemistry

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abstract = "The frit inlet asymmetrical flow field-flow fractionation is applied to the separation of proteins by using a stopless flow injection procedure. By utilizing a small permeable frit near the injection point in an asymmetrical flow FFF channel, sample materials injected to the flow streams can be hydrodynamically relaxed by the compressing action of high speed frit flow and the focusing/relaxation procedure can be bypassed. The separation efficiency of the frit inlet asymmetrical flow FFF channel is demonstrated with few protein standards by examining the influence of the ratio of injection flow rate to frit flow rate on the band broadening during relaxation.",

author = "Moon, {Myeong Hee} and Hansun Kwon and Ilyong Park",

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AU - Kwon, Hansun

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Y1 - 1997

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