Stopping Autoinducer-2 Chatter by Means of an Indigenous Bacterium (Acinetobacter sp. DKY-1): A New Antibiofouling Strategy in a Membrane Bioreactor for Wastewater Treatment

Kibaek Lee, Yea Won Kim, Seonki Lee, Sang Hyun Lee, Chang Hyun Nahm, Hyeokpil Kwon, Pyung Kyu Park, Kwang Ho Choo, Ismail Koyuncu, Anja Drews, Chung Hak Lee, Jung Kee Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bacterial quorum quenching (QQ) by means of degrading signaling molecules has been applied to antibiofouling strategies in a membrane bioreactor (MBR) for wastewater treatment. However, the target signaling molecules have been limited to N-acyl homoserine lactones participating in intraspecies quorum sensing. Here, an approach to disrupting autoinducer-2 (AI-2) signaling molecules participating in interspecies quorum sensing was pursued as a next-generation antibiofouling strategy in an MBR for wastewater treatment. We isolated an indigenous QQ bacterium (Acinetobacter sp. DKY-1) that can attenuate the expression of the quorum-sensing (QS) response through the inactivation of an autoinducer-2 signaling molecule, 4,5-dihydroxy-2,3-pentanedione (DPD), among four kinds of autoinducer-2 QS bacteria. DKY-1 released AI-2 QQ compounds, which were verified to be hydrophilic with a molecular weight of <400 Da. The addition of DKY-1 entrapping beads into an MBR significantly decreased DPD concentration and remarkably reduced membrane biofouling. This new approach, combining molecular biology with wastewater engineering, could enlarge the range of QQ-MBR for antibiofouling and energy savings in the field of wastewater treatment.

Original languageEnglish
Pages (from-to)6237-6245
Number of pages9
JournalEnvironmental Science and Technology
Volume52
Issue number11
DOIs
Publication statusPublished - 2018 Jun 5

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

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