TY - JOUR
T1 - Stopping Autoinducer-2 Chatter by Means of an Indigenous Bacterium (Acinetobacter sp. DKY-1)
T2 - A New Antibiofouling Strategy in a Membrane Bioreactor for Wastewater Treatment
AU - Lee, Kibaek
AU - Kim, Yea Won
AU - Lee, Seonki
AU - Lee, Sang Hyun
AU - Nahm, Chang Hyun
AU - Kwon, Hyeokpil
AU - Park, Pyung Kyu
AU - Choo, Kwang Ho
AU - Koyuncu, Ismail
AU - Drews, Anja
AU - Lee, Chung Hak
AU - Lee, Jung Kee
N1 - Funding Information:
This work was supported through the Korea Ministry of Environment (MOE) and the Convergence Technology Program (grant no. 2015001640001).
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/6/5
Y1 - 2018/6/5
N2 - Bacterial quorum quenching (QQ) by means of degrading signaling molecules has been applied to antibiofouling strategies in a membrane bioreactor (MBR) for wastewater treatment. However, the target signaling molecules have been limited to N-acyl homoserine lactones participating in intraspecies quorum sensing. Here, an approach to disrupting autoinducer-2 (AI-2) signaling molecules participating in interspecies quorum sensing was pursued as a next-generation antibiofouling strategy in an MBR for wastewater treatment. We isolated an indigenous QQ bacterium (Acinetobacter sp. DKY-1) that can attenuate the expression of the quorum-sensing (QS) response through the inactivation of an autoinducer-2 signaling molecule, 4,5-dihydroxy-2,3-pentanedione (DPD), among four kinds of autoinducer-2 QS bacteria. DKY-1 released AI-2 QQ compounds, which were verified to be hydrophilic with a molecular weight of <400 Da. The addition of DKY-1 entrapping beads into an MBR significantly decreased DPD concentration and remarkably reduced membrane biofouling. This new approach, combining molecular biology with wastewater engineering, could enlarge the range of QQ-MBR for antibiofouling and energy savings in the field of wastewater treatment.
AB - Bacterial quorum quenching (QQ) by means of degrading signaling molecules has been applied to antibiofouling strategies in a membrane bioreactor (MBR) for wastewater treatment. However, the target signaling molecules have been limited to N-acyl homoserine lactones participating in intraspecies quorum sensing. Here, an approach to disrupting autoinducer-2 (AI-2) signaling molecules participating in interspecies quorum sensing was pursued as a next-generation antibiofouling strategy in an MBR for wastewater treatment. We isolated an indigenous QQ bacterium (Acinetobacter sp. DKY-1) that can attenuate the expression of the quorum-sensing (QS) response through the inactivation of an autoinducer-2 signaling molecule, 4,5-dihydroxy-2,3-pentanedione (DPD), among four kinds of autoinducer-2 QS bacteria. DKY-1 released AI-2 QQ compounds, which were verified to be hydrophilic with a molecular weight of <400 Da. The addition of DKY-1 entrapping beads into an MBR significantly decreased DPD concentration and remarkably reduced membrane biofouling. This new approach, combining molecular biology with wastewater engineering, could enlarge the range of QQ-MBR for antibiofouling and energy savings in the field of wastewater treatment.
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U2 - 10.1021/acs.est.7b05824
DO - 10.1021/acs.est.7b05824
M3 - Article
C2 - 29714471
AN - SCOPUS:85046533975
SN - 0013-936X
VL - 52
SP - 6237
EP - 6245
JO - Environmental Science & Technology
JF - Environmental Science & Technology
IS - 11
ER -