STR-33, a novel G protein-coupled receptor that regulates locomotion and egg laying in Caenorhabditis elegans

Jeong Eui Lee, Pan Young Jeong, Hyoe Jin Joo, Heekyeong Kim, Taehoon Lee, Hyeon Sook Koo, Young Ki Paik

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Despite their predicted functional importance, most G protein- coupled receptors (GPCRs) in Caenorhabditis elegans have remained largely uncharacterized. Here, we focused on one GPCR, STR-33, encoded by the str-33 gene, which was discovered through a ligand-based screening procedure. To characterize STR-33 function, we performed UV-trimethylpsolaren mutagenesis and isolated an str-33-null mutant. The resulting mutant showed hypersinusoidal movement and a hyperactive egg-laying phenotype. Two types of egg laying-related mutations have been characterized: egg laying-deficient (Egl-d) and hyperactive egg laying (Egl-c). The defect responsible for the egg laying-deficient Egl-d phenotype is related to Gα q signaling, whereas that responsible for the opposite, hyperactive egg-laying Egl-c phenotype is related to Gα o signaling. We found that the hyperactive egg-laying defect of the str-33(ykp001) mutant is dependent on the G protein GOA-1/ Gα o. Endogenous acetylcholine suppressed egg laying in C. elegans via a Gα o-signaling pathway by inhibiting serotonin biosynthesis or release from the hermaphrodite-specific neuron. Consistent with this, in vivo expression of the serotonin biosynthetic enzyme, TPH-1, was up-regulated in the str-33(ykp001) mutant. Taken together, these results suggest that the GPCR, STR-33, may be one of the neurotransmitter receptors that regulates locomotion and egg laying in C. elegans.

Original languageEnglish
Pages (from-to)39860-39870
Number of pages11
JournalJournal of Biological Chemistry
Volume286
Issue number46
DOIs
Publication statusPublished - 2011 Nov 18

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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