Stratum corneum acidification is impaired in moderately aged human and murine skin

Eung Ho Choi, Mao Qiang Man, Pu Xu, Shujun Xin, Zhili Liu, Debra A. Crumrine, Yan J. Jiang, Joachim W. Fluhr, Kenneth R. Feingold, Peter M. Elias, Theodora M. Mauro

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Aged skin commonly is afflicted by inflammatory skin diseases or xerosis/eczema that could be triggered or exacerbated by impaired epidermal permeability barrier homeostasis. This defect is linked to reduced epidermal lipid synthesis in humans and in mice of advanced age (i.e., >75 years in human or >18-24 months in mice). We now report that barrier defects in moderately aged humans (50-80 years) or analogously aged mice (12-15 months) are linked instead to defective stratum corneum (SC) acidity. In moderately aged mouse epidermis, we find that abnormal acidification, in turn, is linked to decreased Na+/H+ antiporter (NHE1) expression. Decreased NHE1 levels lead to increased SC pH, which results in defective lipid processing and delayed maturation of lamellar membranes, due to suboptimal activation of the pH-sensitive essential, lipid-processing enzyme, β-glucocerebrosidase. Conversely, impaired SC integrity in moderately aged mice is due to increased pH-dependent activation of serine proteases, leading to premature degradation of corneodesmosomes. These abnormalities were normalized by exogenously acidifying the SC, suggesting a basis for the well-known acidification therapies that are widely used to treat the pathologic xerosis/eczema seen in moderately aged humans.

Original languageEnglish
Pages (from-to)2847-2856
Number of pages10
JournalJournal of Investigative Dermatology
Volume127
Issue number12
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Acidification
Cornea
Skin
Lipids
Chemical activation
Glucosylceramidase
Eczema
Defects
Sodium-Hydrogen Antiporter
Serine Proteases
Processing
Acidity
Membranes
Degradation
Skin Diseases
Epidermis
Enzymes
Permeability
Homeostasis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Choi, Eung Ho ; Man, Mao Qiang ; Xu, Pu ; Xin, Shujun ; Liu, Zhili ; Crumrine, Debra A. ; Jiang, Yan J. ; Fluhr, Joachim W. ; Feingold, Kenneth R. ; Elias, Peter M. ; Mauro, Theodora M. / Stratum corneum acidification is impaired in moderately aged human and murine skin. In: Journal of Investigative Dermatology. 2007 ; Vol. 127, No. 12. pp. 2847-2856.
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abstract = "Aged skin commonly is afflicted by inflammatory skin diseases or xerosis/eczema that could be triggered or exacerbated by impaired epidermal permeability barrier homeostasis. This defect is linked to reduced epidermal lipid synthesis in humans and in mice of advanced age (i.e., >75 years in human or >18-24 months in mice). We now report that barrier defects in moderately aged humans (50-80 years) or analogously aged mice (12-15 months) are linked instead to defective stratum corneum (SC) acidity. In moderately aged mouse epidermis, we find that abnormal acidification, in turn, is linked to decreased Na+/H+ antiporter (NHE1) expression. Decreased NHE1 levels lead to increased SC pH, which results in defective lipid processing and delayed maturation of lamellar membranes, due to suboptimal activation of the pH-sensitive essential, lipid-processing enzyme, β-glucocerebrosidase. Conversely, impaired SC integrity in moderately aged mice is due to increased pH-dependent activation of serine proteases, leading to premature degradation of corneodesmosomes. These abnormalities were normalized by exogenously acidifying the SC, suggesting a basis for the well-known acidification therapies that are widely used to treat the pathologic xerosis/eczema seen in moderately aged humans.",
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Choi, EH, Man, MQ, Xu, P, Xin, S, Liu, Z, Crumrine, DA, Jiang, YJ, Fluhr, JW, Feingold, KR, Elias, PM & Mauro, TM 2007, 'Stratum corneum acidification is impaired in moderately aged human and murine skin', Journal of Investigative Dermatology, vol. 127, no. 12, pp. 2847-2856. https://doi.org/10.1038/sj.jid.5700913

Stratum corneum acidification is impaired in moderately aged human and murine skin. / Choi, Eung Ho; Man, Mao Qiang; Xu, Pu; Xin, Shujun; Liu, Zhili; Crumrine, Debra A.; Jiang, Yan J.; Fluhr, Joachim W.; Feingold, Kenneth R.; Elias, Peter M.; Mauro, Theodora M.

In: Journal of Investigative Dermatology, Vol. 127, No. 12, 01.01.2007, p. 2847-2856.

Research output: Contribution to journalArticle

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AU - Mauro, Theodora M.

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