Abstract
Methylovorus sp. strain SS1 DSM 11726 was found to grow continuously when it was transferred from 30°C to 40°C and 43°C. A shift in growth temperature from 30°C to 45°C, 47°C and 50°C reduced the viability of the cell population by more than 102, 103 and 105 folds, respectively, after l h cultivation. Cells transferred to 47°C and 50°C after preincubation for 15 min at 43°C, however, exhibited 10-fold increase in viability. It was found that incubation for 15 min at 40°C of Methylovorus sp. strain SS1 grown at 30°C was sufficient to accelerate the synthesis of a specific subset of proteins. The major heat shock proteins had apparent molecular masses of 90,70, 66, 60, and 58 kDa. The 60 and 58 kDa proteins were found to cross-react with the antiserum raised against GroEL protein. The heat shock response persisted for over 1 h. The shock proteins were stable for 90 min in the cell. Exposure of the cells to methanol induced proteins identical to the heat shock proteins. Addition of ethanol induced a unique protein with a molecular mass of about 40 kDa in addition to the heat- induced proteins. The proteins induced in paraquat-treated cells were different from the heat shock proteins, except the 70 and 60 kDa proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 162-167 |
| Number of pages | 6 |
| Journal | Journal of Microbiology |
| Volume | 39 |
| Issue number | 3 |
| Publication status | Published - 2001 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Applied Microbiology and Biotechnology
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Stress-shock Response of a Methylotrophic Bacterium Methylovorus sp. strain SS1 DSM 11726. / Park, Jong H.; Kim, Si W.; KIm, Eungbin; Ro, Young T.; Kim, Young M.
In: Journal of Microbiology, Vol. 39, No. 3, 01.12.2001, p. 162-167.Research output: Contribution to journal › Article
TY - JOUR
T1 - Stress-shock Response of a Methylotrophic Bacterium Methylovorus sp. strain SS1 DSM 11726
AU - Park, Jong H.
AU - Kim, Si W.
AU - KIm, Eungbin
AU - Ro, Young T.
AU - Kim, Young M.
PY - 2001/12/1
Y1 - 2001/12/1
N2 - Methylovorus sp. strain SS1 DSM 11726 was found to grow continuously when it was transferred from 30°C to 40°C and 43°C. A shift in growth temperature from 30°C to 45°C, 47°C and 50°C reduced the viability of the cell population by more than 102, 103 and 105 folds, respectively, after l h cultivation. Cells transferred to 47°C and 50°C after preincubation for 15 min at 43°C, however, exhibited 10-fold increase in viability. It was found that incubation for 15 min at 40°C of Methylovorus sp. strain SS1 grown at 30°C was sufficient to accelerate the synthesis of a specific subset of proteins. The major heat shock proteins had apparent molecular masses of 90,70, 66, 60, and 58 kDa. The 60 and 58 kDa proteins were found to cross-react with the antiserum raised against GroEL protein. The heat shock response persisted for over 1 h. The shock proteins were stable for 90 min in the cell. Exposure of the cells to methanol induced proteins identical to the heat shock proteins. Addition of ethanol induced a unique protein with a molecular mass of about 40 kDa in addition to the heat- induced proteins. The proteins induced in paraquat-treated cells were different from the heat shock proteins, except the 70 and 60 kDa proteins.
AB - Methylovorus sp. strain SS1 DSM 11726 was found to grow continuously when it was transferred from 30°C to 40°C and 43°C. A shift in growth temperature from 30°C to 45°C, 47°C and 50°C reduced the viability of the cell population by more than 102, 103 and 105 folds, respectively, after l h cultivation. Cells transferred to 47°C and 50°C after preincubation for 15 min at 43°C, however, exhibited 10-fold increase in viability. It was found that incubation for 15 min at 40°C of Methylovorus sp. strain SS1 grown at 30°C was sufficient to accelerate the synthesis of a specific subset of proteins. The major heat shock proteins had apparent molecular masses of 90,70, 66, 60, and 58 kDa. The 60 and 58 kDa proteins were found to cross-react with the antiserum raised against GroEL protein. The heat shock response persisted for over 1 h. The shock proteins were stable for 90 min in the cell. Exposure of the cells to methanol induced proteins identical to the heat shock proteins. Addition of ethanol induced a unique protein with a molecular mass of about 40 kDa in addition to the heat- induced proteins. The proteins induced in paraquat-treated cells were different from the heat shock proteins, except the 70 and 60 kDa proteins.
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M3 - Article
VL - 39
SP - 162
EP - 167
JO - Journal of Microbiology
JF - Journal of Microbiology
SN - 1225-8873
IS - 3
ER -