Abstract
Current cell-based therapies administered after myocardial infarction (MI) show limited efficacy due to subpar cell retention in a dynamically beating heart. In particular, cardiac patches generally provide a cursory level of cell attachment due to the lack of an adequate microenvironment. From this perspective, decellularized cell-derived ECM (CDM) is attractive in its recapitulation of a natural biophysical environment for cells. Unfortunately, its weak physical property renders it difficult to retain in its original form, limiting its full potential. Here, a novel strategy to peel CDM off from its underlying substrate is proposed. By physically stamping it onto a polyvinyl alcohol hydrogel, the resulting stretchable extracellular matrix (ECM) membrane preserves the natural microenvironment of CDM, thereby conferring a biological interface to a viscoelastic membrane. Its various mechanical and biological properties are characterized and its capacity to improve cardiomyocyte functionality is demonstrated. Finally, evidence of enhanced stem cell delivery using the stretchable ECM membrane is presented, which leads to improved cardiac remodeling in a rat MI model. A new class of material based on natural CDM is envisioned for the enhanced delivery of cells and growth factors that have a known affinity with ECM.
| Original language | English |
|---|---|
| Article number | 1900593 |
| Journal | Advanced Healthcare Materials |
| Volume | 8 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - 2019 Sep 1 |
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All Science Journal Classification (ASJC) codes
- Biomaterials
- Biomedical Engineering
- Pharmaceutical Science
Cite this
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Stretchable ECM Patch Enhances Stem Cell Delivery for Post-MI Cardiovascular Repair. / Kim, In Gul; Hwang, Mintai P.; Park, Jin Sil; Kim, Su Hyun; Kim, Jung Hyun; Kang, Hyo Jin; Subbiah, Ramesh; Ko, Ung Hyun; Shin, Jennifer H.; Kim, Chong Hyun; Choi, Donghoon; Park, Kwideok.
In: Advanced Healthcare Materials, Vol. 8, No. 17, 1900593, 01.09.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - Stretchable ECM Patch Enhances Stem Cell Delivery for Post-MI Cardiovascular Repair
AU - Kim, In Gul
AU - Hwang, Mintai P.
AU - Park, Jin Sil
AU - Kim, Su Hyun
AU - Kim, Jung Hyun
AU - Kang, Hyo Jin
AU - Subbiah, Ramesh
AU - Ko, Ung Hyun
AU - Shin, Jennifer H.
AU - Kim, Chong Hyun
AU - Choi, Donghoon
AU - Park, Kwideok
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Current cell-based therapies administered after myocardial infarction (MI) show limited efficacy due to subpar cell retention in a dynamically beating heart. In particular, cardiac patches generally provide a cursory level of cell attachment due to the lack of an adequate microenvironment. From this perspective, decellularized cell-derived ECM (CDM) is attractive in its recapitulation of a natural biophysical environment for cells. Unfortunately, its weak physical property renders it difficult to retain in its original form, limiting its full potential. Here, a novel strategy to peel CDM off from its underlying substrate is proposed. By physically stamping it onto a polyvinyl alcohol hydrogel, the resulting stretchable extracellular matrix (ECM) membrane preserves the natural microenvironment of CDM, thereby conferring a biological interface to a viscoelastic membrane. Its various mechanical and biological properties are characterized and its capacity to improve cardiomyocyte functionality is demonstrated. Finally, evidence of enhanced stem cell delivery using the stretchable ECM membrane is presented, which leads to improved cardiac remodeling in a rat MI model. A new class of material based on natural CDM is envisioned for the enhanced delivery of cells and growth factors that have a known affinity with ECM.
AB - Current cell-based therapies administered after myocardial infarction (MI) show limited efficacy due to subpar cell retention in a dynamically beating heart. In particular, cardiac patches generally provide a cursory level of cell attachment due to the lack of an adequate microenvironment. From this perspective, decellularized cell-derived ECM (CDM) is attractive in its recapitulation of a natural biophysical environment for cells. Unfortunately, its weak physical property renders it difficult to retain in its original form, limiting its full potential. Here, a novel strategy to peel CDM off from its underlying substrate is proposed. By physically stamping it onto a polyvinyl alcohol hydrogel, the resulting stretchable extracellular matrix (ECM) membrane preserves the natural microenvironment of CDM, thereby conferring a biological interface to a viscoelastic membrane. Its various mechanical and biological properties are characterized and its capacity to improve cardiomyocyte functionality is demonstrated. Finally, evidence of enhanced stem cell delivery using the stretchable ECM membrane is presented, which leads to improved cardiac remodeling in a rat MI model. A new class of material based on natural CDM is envisioned for the enhanced delivery of cells and growth factors that have a known affinity with ECM.
UR - http://www.scopus.com/inward/record.url?scp=85069748365&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85069748365&partnerID=8YFLogxK
U2 - 10.1002/adhm.201900593
DO - 10.1002/adhm.201900593
M3 - Article
C2 - 31304685
AN - SCOPUS:85069748365
VL - 8
JO - Advanced healthcare materials
JF - Advanced healthcare materials
SN - 2192-2640
IS - 17
M1 - 1900593
ER -