Abstract
Ex vivo induction of cardiomyogenic differentiation of mesenchymal stem cells (MSCs) before implantation would potentiate therapeutic efficacy of stem cell therapies for ischemic heart diseases because MSCs rarely undergo cardiomyogenic differentiation following implantation. In cardiac microenvironments, electric pulse and cyclic mechanical strain are sequentially produced. However, no study has applied the pulsatile mechanoelectric cues (PMEC) to stimulate cardiomyogenic differentiation of MSCs ex vivo. In this study, we developed a stretchable piezoelectric substrate (SPS) that can provide PMEC to human MSCs (hMSCs) for cardiomyogenic differentiation ex vivo. Our data showed that hMSCs subjected to PMEC by SPS underwent promoted cardiac phenotype development: cell alignment and the expression of cardiac markers (i.e., cardiac transcription factors, structural proteins, ion channel proteins, and gap junction proteins). The enhanced cardiac phenotype development was mediated by the upregulation of cardiomyogenic differentiation-related autocrine factor expression, focal adhesion kinase, and extracellular signal-regulated kinases signaling pathways. Thus, SPS providing electrical and mechanical regulation of stem cells may be utilized to potentiate hMSC therapies for myocardial infarction and provide a tool for the study of stem cell biology.
Original language | English |
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Pages (from-to) | 22101-22111 |
Number of pages | 11 |
Journal | ACS Applied Materials and Interfaces |
Volume | 9 |
Issue number | 27 |
DOIs | |
Publication status | Published - 2017 Jul 12 |
Bibliographical note
Funding Information:This study was supported by grants (HI15C0498, HI15C3029 and HI14C1550) from the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, a grant (2017R1A2B3005842) from the National Research Foundation of Korea, and the LG Display academic industrial cooperation program.
Publisher Copyright:
© 2017 American Chemical Society.
All Science Journal Classification (ASJC) codes
- Materials Science(all)