Stromal and epithelial caveolin-1 both confer a protective effect against mammary hyperplasia and tumorigenesis: Caveolin-1 antagonizes cyclin D1 function in mammary epithelial cells

Terence M. Williams, Federica Sotgia, Hyangkyu Lee, Ghada Hassan, Dolores Di Vizio, Gloria Bonuccelli, Franco Capozza, Isabelle Mercier, Hallgeir Rui, Richard G. Pestell, Michael P. Lisanti

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Abstract

Here, we investigate the role of caveolin-1 (Cav-1) in breast cancer onset and progression, with a focus on epithelial-stromal interactions, ie, the tumor microenvironment. Cav-1 is highly expressed in adipocytes and is abundant in mammary fat pads (stroma), but it remains unknown whether loss of Cav-1 within mammary stromal cells affects the differentiated state of mammary epithelia via paracrine signaling. To address this issue, we characterized the development of the mammary ductal system in Cav-1-/- mice and performed a series of mammary transplant studies, using both wild-type and Cav-1-/- mammary fat pads. Cav-1-/- mammary epithelia were hyperproliferative in vivo, with dramatic increases in terminal end bud area and mammary ductal thickness as well as increases in bromodeoxyuridine incorporation, extracellular signal-regulated kinase-1/2 hyperactivation, and up-regulation of STAT5a and cyclin D1. Consistent with these findings, loss of Cav-1 dramatically exacerbated mammary lobulo-alveolar hyperplasia in cyclin D1 Tg mice, whereas overexpression of Cav-1 caused reversion of this phenotype. Most importantly, Cav-1-/- mammary stromal cells (fat pads) promoted the growth of both normal mammary ductal epithelia and mammary tumor cells. Thus, Cav-1 expression in both epithelial and stromal cells provides a protective effect against mammary hyperplasia as well as mammary tumorigenesis.

Original languageEnglish
Pages (from-to)1784-1801
Number of pages18
JournalAmerican Journal of Pathology
Volume169
Issue number5
DOIs
Publication statusPublished - 2006 Nov

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Caveolin 1
Cyclin D1
Hyperplasia
Carcinogenesis
Breast
Epithelial Cells
Stromal Cells
Adipose Tissue
Epithelium
Paracrine Communication
Breast Neoplasms
Tumor Microenvironment
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Bromodeoxyuridine
Adipocytes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Williams, Terence M. ; Sotgia, Federica ; Lee, Hyangkyu ; Hassan, Ghada ; Di Vizio, Dolores ; Bonuccelli, Gloria ; Capozza, Franco ; Mercier, Isabelle ; Rui, Hallgeir ; Pestell, Richard G. ; Lisanti, Michael P. / Stromal and epithelial caveolin-1 both confer a protective effect against mammary hyperplasia and tumorigenesis : Caveolin-1 antagonizes cyclin D1 function in mammary epithelial cells. In: American Journal of Pathology. 2006 ; Vol. 169, No. 5. pp. 1784-1801.
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Stromal and epithelial caveolin-1 both confer a protective effect against mammary hyperplasia and tumorigenesis : Caveolin-1 antagonizes cyclin D1 function in mammary epithelial cells. / Williams, Terence M.; Sotgia, Federica; Lee, Hyangkyu; Hassan, Ghada; Di Vizio, Dolores; Bonuccelli, Gloria; Capozza, Franco; Mercier, Isabelle; Rui, Hallgeir; Pestell, Richard G.; Lisanti, Michael P.

In: American Journal of Pathology, Vol. 169, No. 5, 11.2006, p. 1784-1801.

Research output: Contribution to journalArticle

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AU - Lee, Hyangkyu

AU - Hassan, Ghada

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AU - Bonuccelli, Gloria

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AU - Mercier, Isabelle

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AU - Pestell, Richard G.

AU - Lisanti, Michael P.

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