Stromal fibroblasts from the interface zone of human breast carcinomas induce an epithelial-mesenchymal transition-like state in breast cancer cells in vitro

Ming Qing Gao, Baek Gil Kim, Suki Kang, Yoon Pyo Choi, Hangran Park, Kyu Sub Kang, Nam Hoon Cho

Research output: Contribution to journalArticle

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Abstract

Fibroblasts were extracted from tissue in tumor burden zones, distal normal zones and interface zones between tumor and normal tissue of human breast carcinomas, and the corresponding fibroblasts were designated as cancer-associated fibroblasts (CAFs), normal zone fibroblasts (NFs) and interface zone fibroblasts (INFs). The crosstalk between three types of fibroblasts and breast cancer cells was evaluated using an in vitro direct co-culture model. We found that INFs grew faster and expressed higher levels of fibroblast activation protein than did NFs and CAFs. Compared with CAFs and NFs, INFs grown with breast cancer cells were significantly more effective in inducing an epithelial-mesenchymal transition (EMT) in cancer cells, as indicated by induction of vimentin and N-cadherin and downregulation of E-cadherin. This EMT process was also accompanied by activation of extracellular signal-regulated kinase (ERK) and modulation of membrane-type 1 matrix metalloproteinase (MT1-MMP) expression. Additionally, INFs promoted breast cell migration to a larger extent compared with NFs and CAFs. Taken together, these findings indicate that INFs isolated from the tumor interface zone exhibited more robust biological modulatory activity than did NFs and CAFs isolated from normal and tumor zones of the same tumor tissue, suggesting that the interface zone of the tumor represents a dynamic region vital to tumor progression.

Original languageEnglish
Pages (from-to)3507-3514
Number of pages8
JournalJournal of cell science
Volume123
Issue number20
DOIs
Publication statusPublished - 2010 Oct 15

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Epithelial-Mesenchymal Transition
Fibroblasts
Breast Neoplasms
Neoplasms
Cadherins
In Vitro Techniques
Matrix Metalloproteinase 14
Extracellular Signal-Regulated MAP Kinases
Vimentin
Coculture Techniques
Tumor Burden
Cell Movement

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Gao, Ming Qing ; Kim, Baek Gil ; Kang, Suki ; Choi, Yoon Pyo ; Park, Hangran ; Kang, Kyu Sub ; Cho, Nam Hoon. / Stromal fibroblasts from the interface zone of human breast carcinomas induce an epithelial-mesenchymal transition-like state in breast cancer cells in vitro. In: Journal of cell science. 2010 ; Vol. 123, No. 20. pp. 3507-3514.
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Stromal fibroblasts from the interface zone of human breast carcinomas induce an epithelial-mesenchymal transition-like state in breast cancer cells in vitro. / Gao, Ming Qing; Kim, Baek Gil; Kang, Suki; Choi, Yoon Pyo; Park, Hangran; Kang, Kyu Sub; Cho, Nam Hoon.

In: Journal of cell science, Vol. 123, No. 20, 15.10.2010, p. 3507-3514.

Research output: Contribution to journalArticle

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