Strong contact coupling of neuronal growth cones with height-controlled vertical silicon nanocolumns

Seong Min Kim, Seyeong Lee, Dongyoon Kim, Dong Hee Kang, Kisuk Yang, Seung-Woo Cho, Jin Seok Lee, Insung S. Choi, Kyungtae Kang, Myung Han Yoon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this study, we report that height-controlled vertically etched silicon nano-column arrays (vSNAs) induce strong growth cone-to-substrate coupling and accelerate In vitroneurite development while preserving the essential features of initial neurite formation. Large-scale preparation of vSNAs with flat head morphology enabled the generation of well-controlled topographical stimulation without cellular impalement. A systematic analysis on topography-induced variations on cellular morphology and cytoskeletal dynamics was conducted. In addition, neurite development on the grid-patterned vSNAs exhibited preferential adhesion to the nanostructured region and outgrowth directionality. The arrangement of cytoskeletal proteins and the expression of a focal adhesion complex indicated that a strong coupling existed between the underlying nanocolumns and growth cones. Furthermore, the height-controlled nanocolumn substrates differentially modulated neurite polarization and elongation. Our findings provide an important insight into neuron-nanotopography interactions and their role in cell adhesion and neurite development. [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)2532-2543
Number of pages12
JournalNano Research
Volume11
Issue number5
DOIs
Publication statusPublished - 2018 May 1

Fingerprint

Silicon
Cones
Adhesion
Cytoskeletal Proteins
Cell adhesion
Substrates
Topography
Neurons
Elongation
Polarization
Proteins

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Electrical and Electronic Engineering

Cite this

Kim, Seong Min ; Lee, Seyeong ; Kim, Dongyoon ; Kang, Dong Hee ; Yang, Kisuk ; Cho, Seung-Woo ; Lee, Jin Seok ; Choi, Insung S. ; Kang, Kyungtae ; Yoon, Myung Han. / Strong contact coupling of neuronal growth cones with height-controlled vertical silicon nanocolumns. In: Nano Research. 2018 ; Vol. 11, No. 5. pp. 2532-2543.
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Kim, SM, Lee, S, Kim, D, Kang, DH, Yang, K, Cho, S-W, Lee, JS, Choi, IS, Kang, K & Yoon, MH 2018, 'Strong contact coupling of neuronal growth cones with height-controlled vertical silicon nanocolumns', Nano Research, vol. 11, no. 5, pp. 2532-2543. https://doi.org/10.1007/s12274-017-1878-7

Strong contact coupling of neuronal growth cones with height-controlled vertical silicon nanocolumns. / Kim, Seong Min; Lee, Seyeong; Kim, Dongyoon; Kang, Dong Hee; Yang, Kisuk; Cho, Seung-Woo; Lee, Jin Seok; Choi, Insung S.; Kang, Kyungtae; Yoon, Myung Han.

In: Nano Research, Vol. 11, No. 5, 01.05.2018, p. 2532-2543.

Research output: Contribution to journalArticle

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AU - Lee, Jin Seok

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AU - Kang, Kyungtae

AU - Yoon, Myung Han

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