Strong contact coupling of neuronal growth cones with height-controlled vertical silicon nanocolumns

Seong Min Kim, Seyeong Lee, Dongyoon Kim, Dong Hee Kang, Kisuk Yang, Seung Woo Cho, Jin Seok Lee, Insung S. Choi, Kyungtae Kang, Myung Han Yoon

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5 Citations (Scopus)

Abstract

In this study, we report that height-controlled vertically etched silicon nano-column arrays (vSNAs) induce strong growth cone-to-substrate coupling and accelerate In vitroneurite development while preserving the essential features of initial neurite formation. Large-scale preparation of vSNAs with flat head morphology enabled the generation of well-controlled topographical stimulation without cellular impalement. A systematic analysis on topography-induced variations on cellular morphology and cytoskeletal dynamics was conducted. In addition, neurite development on the grid-patterned vSNAs exhibited preferential adhesion to the nanostructured region and outgrowth directionality. The arrangement of cytoskeletal proteins and the expression of a focal adhesion complex indicated that a strong coupling existed between the underlying nanocolumns and growth cones. Furthermore, the height-controlled nanocolumn substrates differentially modulated neurite polarization and elongation. Our findings provide an important insight into neuron-nanotopography interactions and their role in cell adhesion and neurite development. [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)2532-2543
Number of pages12
JournalNano Research
Volume11
Issue number5
DOIs
Publication statusPublished - 2018 May 1

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Electrical and Electronic Engineering

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    Kim, S. M., Lee, S., Kim, D., Kang, D. H., Yang, K., Cho, S. W., Lee, J. S., Choi, I. S., Kang, K., & Yoon, M. H. (2018). Strong contact coupling of neuronal growth cones with height-controlled vertical silicon nanocolumns. Nano Research, 11(5), 2532-2543. https://doi.org/10.1007/s12274-017-1878-7