Structural and conformational dynamics of self-assembling bioactive β-sheet peptide nanostructures decorated with multivalent RNA-binding peptides

Sanghun Han, Donghun Kim, So Hee Han, Nam Hee Kim, Sun Hee Kim, Yong Beom Lim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Understanding the dynamic behavior of nanostructural systems is important during the development of controllable and tailor-made nanomaterials. This is particularly true for nanostructures that are intended for biological applications because biomolecules are usually highly dynamic and responsive to external stimuli. In this Article, we investigated the structural and conformational dynamics of self-assembling bioactive β-sheet peptide nanostructures using electron paramagnetic resonance (EPR) spectroscopy. The model peptide nanostructures are characterized by the cross-β spine of β-ribbon fibers and multiple RNA-binding bioactive peptides that constitute the shell of the nanostructures. We found first, that bioactive peptides at the shell of β-ribbon nanostructure have a mobility similar to that of an isolated monomeric peptide. Second, the periphery of the cross-β spine is more immobile than the distal part of surface-displayed bioactive peptides. Third, the rotational dynamics of short and long fibrils are similar; that is, the mobility is largely independent of the extent of aggregation. Fourth, peptides that constitute the shell are affected first by the external environment at the initial stage. The cross-β spine resists its external environment to a certain extent and abruptly disintegrates when the perturbation reaches a certain degree. Our results provide an overall picture of β-sheet peptide nanostructure dynamics, which should be useful in the development of dynamic self-assembled peptide nanostructures.

Original languageEnglish
Pages (from-to)16047-16053
Number of pages7
JournalJournal of the American Chemical Society
Volume134
Issue number38
DOIs
Publication statusPublished - 2012 Sep 26

Fingerprint

Nanostructures
RNA
Peptides
Spine
Electron Spin Resonance Spectroscopy
Biomolecules
Nanostructured materials
Paramagnetic resonance
Spectrum Analysis
Agglomeration
Spectroscopy
Fibers

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

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title = "Structural and conformational dynamics of self-assembling bioactive β-sheet peptide nanostructures decorated with multivalent RNA-binding peptides",
abstract = "Understanding the dynamic behavior of nanostructural systems is important during the development of controllable and tailor-made nanomaterials. This is particularly true for nanostructures that are intended for biological applications because biomolecules are usually highly dynamic and responsive to external stimuli. In this Article, we investigated the structural and conformational dynamics of self-assembling bioactive β-sheet peptide nanostructures using electron paramagnetic resonance (EPR) spectroscopy. The model peptide nanostructures are characterized by the cross-β spine of β-ribbon fibers and multiple RNA-binding bioactive peptides that constitute the shell of the nanostructures. We found first, that bioactive peptides at the shell of β-ribbon nanostructure have a mobility similar to that of an isolated monomeric peptide. Second, the periphery of the cross-β spine is more immobile than the distal part of surface-displayed bioactive peptides. Third, the rotational dynamics of short and long fibrils are similar; that is, the mobility is largely independent of the extent of aggregation. Fourth, peptides that constitute the shell are affected first by the external environment at the initial stage. The cross-β spine resists its external environment to a certain extent and abruptly disintegrates when the perturbation reaches a certain degree. Our results provide an overall picture of β-sheet peptide nanostructure dynamics, which should be useful in the development of dynamic self-assembled peptide nanostructures.",
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Structural and conformational dynamics of self-assembling bioactive β-sheet peptide nanostructures decorated with multivalent RNA-binding peptides. / Han, Sanghun; Kim, Donghun; Han, So Hee; Kim, Nam Hee; Kim, Sun Hee; Lim, Yong Beom.

In: Journal of the American Chemical Society, Vol. 134, No. 38, 26.09.2012, p. 16047-16053.

Research output: Contribution to journalArticle

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