Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet /631/45 /631/57 /101 /82 /145 article

Sooho Choi, Pankaj Attri, Inhwan Lee, Jeongmin Oh, Ji Hye Yun, Ji Hoon Park, Eun Ha Choi, Weon Tae Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The variation in the biological function of proteins plays an important role in plasma medicine and sterilization. Several non-thermal plasma sources with different feeding gases are used worldwide for plasma treatment, including dielectric barrier discharge (DBD) and atmospheric-pressure plasma jet (APPJ) as the most commonly used sources. Therefore, in the present work, we used both DBD and APPJ plasma sources with N 2 and air as feeding gases to evaluate the effects on the structural, thermodynamic, and activity changes of enzymes. In the current work, we used lysozyme as a model enzyme and verified the structural changes using circular dichroism (CD), fluorescence, and X-ray crystallography. In addition, we investigated the lysozyme thermodynamics using CD thermal analysis and changes in the B-factor from X-ray crystallography. The results showed that lysozyme activity decreased after the plasma treatment. From these analyses, we concluded that N 2 -feeding gas plasma disturbs the structure and activity of lysozyme more than Air feeding gas plasma in our experimental studies. This study provides novel fundamental information on the changes to enzymes upon plasma treatment, which has been absent from the literature until now.

Original languageEnglish
Article number1027
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Atmospheric Pressure
Muramidase
Plasma Gases
X Ray Crystallography
Circular Dichroism
Thermodynamics
Enzymes
Gases
Air
Factor X
Hot Temperature
Fluorescence
Medicine

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet /631/45 /631/57 /101 /82 /145 article",
abstract = "The variation in the biological function of proteins plays an important role in plasma medicine and sterilization. Several non-thermal plasma sources with different feeding gases are used worldwide for plasma treatment, including dielectric barrier discharge (DBD) and atmospheric-pressure plasma jet (APPJ) as the most commonly used sources. Therefore, in the present work, we used both DBD and APPJ plasma sources with N 2 and air as feeding gases to evaluate the effects on the structural, thermodynamic, and activity changes of enzymes. In the current work, we used lysozyme as a model enzyme and verified the structural changes using circular dichroism (CD), fluorescence, and X-ray crystallography. In addition, we investigated the lysozyme thermodynamics using CD thermal analysis and changes in the B-factor from X-ray crystallography. The results showed that lysozyme activity decreased after the plasma treatment. From these analyses, we concluded that N 2 -feeding gas plasma disturbs the structure and activity of lysozyme more than Air feeding gas plasma in our experimental studies. This study provides novel fundamental information on the changes to enzymes upon plasma treatment, which has been absent from the literature until now.",
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Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet /631/45 /631/57 /101 /82 /145 article. / Choi, Sooho; Attri, Pankaj; Lee, Inhwan; Oh, Jeongmin; Yun, Ji Hye; Park, Ji Hoon; Choi, Eun Ha; Lee, Weon Tae.

In: Scientific reports, Vol. 7, No. 1, 1027, 01.12.2017.

Research output: Contribution to journalArticle

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AU - Oh, Jeongmin

AU - Yun, Ji Hye

AU - Park, Ji Hoon

AU - Choi, Eun Ha

AU - Lee, Weon Tae

PY - 2017/12/1

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