Structural basis for the inhibition of human lysozyme by PliC from Brucella abortus

Si Hyeon Um, Jin Sik Kim, Kuglae Kim, Nahee Kim, Hyun Soo Cho, Nam Chul Ha

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Lysozymes are the first line of defense for a diverse range of organisms that catalyze the degradation of bacterial peptidoglycan. Gram-negative bacteria produce proteinaceous lysozyme inhibitors to protect themselves from the action of lysozymes. To date, MliC or PliC (membrane-bound or periplasmic inhibitor of c-type lysozyme, respectively) has been found in various Gram-negative bacteria. Here, we report the crystal structures of Brucella abortus PliC and its complex with human c-type lysozyme. The complex structure demonstrates that the invariant loop of MliC/PliC plays a crucial role in the inhibition of lysozyme via its insertion into the active site cleft of the lysozyme, as previously observed in the complex structure of Pseudomonas aeruginosa MliC and chicken c-type lysozyme. We identified a new binding interface between a loop adjacent to the active site of human lysozyme and a loop carrying Glu112 of B. abortus PliC, the structure of which was disordered in P. aeruginosa MliC. Because MliC/PliC family members have been implicated as putative colonization or virulence factors, the structures and mechanism of action of MliC/PliC will be relevant to the control of bacterial growth in animal hosts.

Original languageEnglish
Pages (from-to)9385-9393
Number of pages9
JournalBiochemistry
Volume52
Issue number51
DOIs
Publication statusPublished - 2013 Dec 23

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Brucella abortus
Muramidase
Gram-Negative Bacteria
Pseudomonas aeruginosa
Catalytic Domain
Bacteria
Inhibition (Psychology)
Peptidoglycan
Virulence Factors
Chickens
Animals
Crystal structure
Membranes
Degradation

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Um, Si Hyeon ; Kim, Jin Sik ; Kim, Kuglae ; Kim, Nahee ; Cho, Hyun Soo ; Ha, Nam Chul. / Structural basis for the inhibition of human lysozyme by PliC from Brucella abortus. In: Biochemistry. 2013 ; Vol. 52, No. 51. pp. 9385-9393.
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Structural basis for the inhibition of human lysozyme by PliC from Brucella abortus. / Um, Si Hyeon; Kim, Jin Sik; Kim, Kuglae; Kim, Nahee; Cho, Hyun Soo; Ha, Nam Chul.

In: Biochemistry, Vol. 52, No. 51, 23.12.2013, p. 9385-9393.

Research output: Contribution to journalArticle

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