Structural basis for the recognition of lysozyme by MliC, a periplasmic lysozyme inhibitor in Gram-negative bacteria

Soohwan Yum, Moon Jong Kim, Yongbin Xu, Xiao Ling Jin, Hee Young Yoo, Ji Won Park, Ji Hee Gong, Kwang Min Choe, Bok Luel Lee, Nam Chul Ha

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Lysozymes are an important component of the innate immune system of animals that hydrolyze peptidoglycan, the major bacterial cell wall constituent. Many bacteria have contrived various means of dealing with this bactericidal enzyme, one of which is to produce lysozyme inhibitors. Recently, a novel family of bacterial lysozyme inhibitors was identified in various Gram-negative bacteria, named MliC (membrane bound lysozyme inhibitor of C-type lysozyme). Here, we report the crystal structure of Pseudomonas aeruginosa MliC in complex with chicken egg white lysozyme. Combined with mutational study, the complex structure demonstrates that the invariant loop of MliC plays a crucial role in the inhibition of the lysozyme by its insertion to the active site cleft of the lysozyme, where the loop forms hydrogen and ionic bonds with the catalytic residues. Since MliC family members have been implicated as putative colonization or virulence factors, the structures and mechanism of action of MliC will be of relevance to the control of bacterial growth in animal hosts.

Original languageEnglish
Pages (from-to)244-248
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume378
Issue number2
DOIs
Publication statusPublished - 2009 Jan 9

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Muramidase
Gram-Negative Bacteria
Bacteria
Animals
Egg White
Peptidoglycan
Recognition (Psychology)
Immune system
Virulence Factors
Cell Wall
Pseudomonas aeruginosa
Hydrogen
Chickens
Immune System
Catalytic Domain
Crystal structure
Cells
Membranes
Enzymes
Growth

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yum, Soohwan ; Kim, Moon Jong ; Xu, Yongbin ; Jin, Xiao Ling ; Yoo, Hee Young ; Park, Ji Won ; Gong, Ji Hee ; Choe, Kwang Min ; Lee, Bok Luel ; Ha, Nam Chul. / Structural basis for the recognition of lysozyme by MliC, a periplasmic lysozyme inhibitor in Gram-negative bacteria. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 378, No. 2. pp. 244-248.
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Structural basis for the recognition of lysozyme by MliC, a periplasmic lysozyme inhibitor in Gram-negative bacteria. / Yum, Soohwan; Kim, Moon Jong; Xu, Yongbin; Jin, Xiao Ling; Yoo, Hee Young; Park, Ji Won; Gong, Ji Hee; Choe, Kwang Min; Lee, Bok Luel; Ha, Nam Chul.

In: Biochemical and Biophysical Research Communications, Vol. 378, No. 2, 09.01.2009, p. 244-248.

Research output: Contribution to journalArticle

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