Structure-activity relationships of polyhydroxyursane-type triterpenoids on the cytoprotective and anti-inflammatory effects

Hyun Ju Jung, Jung Hwan Nam, Kyung Tae Lee, Yong Sup Lee, Jongwon Choi, Won Bae Kim, WonYoon Chung, Kyun Park Kwang, Hee Juhn Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Eleven polyhydroxyursane triterpenoids (PHUTs) were tested to determine their cytoprotective, immunosuppressive and anti-inflammatory effects. To compare the bioactivities of 19α-hydroxyursane-type triterpenoids {23-hydroxytormentic acid (6), its methyl ester (7), tormentic acid (8), niga-ichigoside F1 (9), euscaphic acid (10) and kaji-ichigoside F1 (11)} of the Rosaceae crude drugs (Rubi Fructus and Rosa rugosae Radix) with PHUTs possessing no 19α-hydroxyl of Centella asiatica (Umbelliferae), the four PHUTs, asiaticoside (1), madecassoside (2), asiatic acid (3), and madecassic acid (4) were isolated from C. asiatica and 23-hydroxyursolic acid (5) from Cussonia bancoensis. Cytoprotective effects were assessed by measuring cell viabilities against cisplatin-induced cytotoxocity in LLC-PK1 cells (proximal tubule, pig kidney) to determine whether these agents have protective effects against nephrotoxicity caused by cisplatin. The inhibitory effect of 11 PHUTs on nitric oxide (NO) and prostaglandin E 2 (PGE2) were evaluated by measuring nitrite accumulation in lipopolysaccharide (LPS)-induced macrophage RAW 264.7 cells, and their anti-inflammatory effects were tested in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema model. Six MHUTs (compounds 1, 2, 4, 6, 10, and 11) exhibited higher cell viabilities during cisplatin-induced cytotoxicity testing even at a concentration of 200 ng/ml than cisplatin only-treated group, suggesting that these compounds have the potent cytoprotective effects. Compounds 1 and 3 of the C. asiatica and niga-ichigoside F1 exhibited no inhibitory effect on NO and/or PGE2 production whereas other PHUTs produced mild to significant NO and/or PGE2 production. The four compounds (2, 5, 9, and 10) potently inhibited mouse ear edema induced by TPA whereas two compounds (1 and 3) had no activity in this test. These results suggest that many PHUTs are potent chemopreventives. Structure-activity relationship (SAR) was also discussed in each assay with regard to the significant role of OHs at the position of 2, 3, 6, 19, and 23 and to the glycoside linkage at the 28-carboxyl.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalNatural Product Sciences
Volume13
Issue number1
Publication statusPublished - 2007 Mar 1

Fingerprint

Centella
Structure-Activity Relationship
Cisplatin
Anti-Inflammatory Agents
Prostaglandins E
Nitric Oxide
Tetradecanoylphorbol Acetate
Ear
Edema
Cell Survival
Acetates
Araliaceae
Cells
LLC-PK1 Cells
Apiaceae
Rosaceae
Protective Agents
Proximal Kidney Tubule
Macrophages
Immunosuppressive Agents

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Organic Chemistry

Cite this

Jung, H. J., Nam, J. H., Lee, K. T., Lee, Y. S., Choi, J., Kim, W. B., ... Park, H. J. (2007). Structure-activity relationships of polyhydroxyursane-type triterpenoids on the cytoprotective and anti-inflammatory effects. Natural Product Sciences, 13(1), 33-39.
Jung, Hyun Ju ; Nam, Jung Hwan ; Lee, Kyung Tae ; Lee, Yong Sup ; Choi, Jongwon ; Kim, Won Bae ; Chung, WonYoon ; Kwang, Kyun Park ; Park, Hee Juhn. / Structure-activity relationships of polyhydroxyursane-type triterpenoids on the cytoprotective and anti-inflammatory effects. In: Natural Product Sciences. 2007 ; Vol. 13, No. 1. pp. 33-39.
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abstract = "Eleven polyhydroxyursane triterpenoids (PHUTs) were tested to determine their cytoprotective, immunosuppressive and anti-inflammatory effects. To compare the bioactivities of 19α-hydroxyursane-type triterpenoids {23-hydroxytormentic acid (6), its methyl ester (7), tormentic acid (8), niga-ichigoside F1 (9), euscaphic acid (10) and kaji-ichigoside F1 (11)} of the Rosaceae crude drugs (Rubi Fructus and Rosa rugosae Radix) with PHUTs possessing no 19α-hydroxyl of Centella asiatica (Umbelliferae), the four PHUTs, asiaticoside (1), madecassoside (2), asiatic acid (3), and madecassic acid (4) were isolated from C. asiatica and 23-hydroxyursolic acid (5) from Cussonia bancoensis. Cytoprotective effects were assessed by measuring cell viabilities against cisplatin-induced cytotoxocity in LLC-PK1 cells (proximal tubule, pig kidney) to determine whether these agents have protective effects against nephrotoxicity caused by cisplatin. The inhibitory effect of 11 PHUTs on nitric oxide (NO) and prostaglandin E 2 (PGE2) were evaluated by measuring nitrite accumulation in lipopolysaccharide (LPS)-induced macrophage RAW 264.7 cells, and their anti-inflammatory effects were tested in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema model. Six MHUTs (compounds 1, 2, 4, 6, 10, and 11) exhibited higher cell viabilities during cisplatin-induced cytotoxicity testing even at a concentration of 200 ng/ml than cisplatin only-treated group, suggesting that these compounds have the potent cytoprotective effects. Compounds 1 and 3 of the C. asiatica and niga-ichigoside F1 exhibited no inhibitory effect on NO and/or PGE2 production whereas other PHUTs produced mild to significant NO and/or PGE2 production. The four compounds (2, 5, 9, and 10) potently inhibited mouse ear edema induced by TPA whereas two compounds (1 and 3) had no activity in this test. These results suggest that many PHUTs are potent chemopreventives. Structure-activity relationship (SAR) was also discussed in each assay with regard to the significant role of OHs at the position of 2, 3, 6, 19, and 23 and to the glycoside linkage at the 28-carboxyl.",
author = "Jung, {Hyun Ju} and Nam, {Jung Hwan} and Lee, {Kyung Tae} and Lee, {Yong Sup} and Jongwon Choi and Kim, {Won Bae} and WonYoon Chung and Kwang, {Kyun Park} and Park, {Hee Juhn}",
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Jung, HJ, Nam, JH, Lee, KT, Lee, YS, Choi, J, Kim, WB, Chung, W, Kwang, KP & Park, HJ 2007, 'Structure-activity relationships of polyhydroxyursane-type triterpenoids on the cytoprotective and anti-inflammatory effects', Natural Product Sciences, vol. 13, no. 1, pp. 33-39.

Structure-activity relationships of polyhydroxyursane-type triterpenoids on the cytoprotective and anti-inflammatory effects. / Jung, Hyun Ju; Nam, Jung Hwan; Lee, Kyung Tae; Lee, Yong Sup; Choi, Jongwon; Kim, Won Bae; Chung, WonYoon; Kwang, Kyun Park; Park, Hee Juhn.

In: Natural Product Sciences, Vol. 13, No. 1, 01.03.2007, p. 33-39.

Research output: Contribution to journalArticle

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N2 - Eleven polyhydroxyursane triterpenoids (PHUTs) were tested to determine their cytoprotective, immunosuppressive and anti-inflammatory effects. To compare the bioactivities of 19α-hydroxyursane-type triterpenoids {23-hydroxytormentic acid (6), its methyl ester (7), tormentic acid (8), niga-ichigoside F1 (9), euscaphic acid (10) and kaji-ichigoside F1 (11)} of the Rosaceae crude drugs (Rubi Fructus and Rosa rugosae Radix) with PHUTs possessing no 19α-hydroxyl of Centella asiatica (Umbelliferae), the four PHUTs, asiaticoside (1), madecassoside (2), asiatic acid (3), and madecassic acid (4) were isolated from C. asiatica and 23-hydroxyursolic acid (5) from Cussonia bancoensis. Cytoprotective effects were assessed by measuring cell viabilities against cisplatin-induced cytotoxocity in LLC-PK1 cells (proximal tubule, pig kidney) to determine whether these agents have protective effects against nephrotoxicity caused by cisplatin. The inhibitory effect of 11 PHUTs on nitric oxide (NO) and prostaglandin E 2 (PGE2) were evaluated by measuring nitrite accumulation in lipopolysaccharide (LPS)-induced macrophage RAW 264.7 cells, and their anti-inflammatory effects were tested in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema model. Six MHUTs (compounds 1, 2, 4, 6, 10, and 11) exhibited higher cell viabilities during cisplatin-induced cytotoxicity testing even at a concentration of 200 ng/ml than cisplatin only-treated group, suggesting that these compounds have the potent cytoprotective effects. Compounds 1 and 3 of the C. asiatica and niga-ichigoside F1 exhibited no inhibitory effect on NO and/or PGE2 production whereas other PHUTs produced mild to significant NO and/or PGE2 production. The four compounds (2, 5, 9, and 10) potently inhibited mouse ear edema induced by TPA whereas two compounds (1 and 3) had no activity in this test. These results suggest that many PHUTs are potent chemopreventives. Structure-activity relationship (SAR) was also discussed in each assay with regard to the significant role of OHs at the position of 2, 3, 6, 19, and 23 and to the glycoside linkage at the 28-carboxyl.

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