Structure and expression of OsMRE11 in rice

Jong Pil Hong, Mi Kim Sang, Young Ryu Moon, Sunghwa Choe, Bum Park Phun, Gynheung An, Woo Taek Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In yeast and human cells, the Mre11 complex, which consists of Mre11, Rad50, and Xrs2/Nbs1 proteins, participates in basic aspects of chromosome metabolism, such as the repair of meiotic DNA breaks and telomere maintenance. In this study, we isolated a full-length cDNA clone, pOsMre11, encoding a rice ortholog of the Mre11 protein. Its predicted protein sequence (Mr = 79.2 kDa and pl value = 5.91) contains a metallo-phosphoesterase domain at its N-terminal region, and a single putative DNA binding domain in the central region of the protein, with significant homology to corresponding motifs in human and yeast Mre11 proteins. The OsMRE11 gene is constitutively expressed in all tissues examined here, including leaves, roots, tillers, and meristems, as well as in undifferentiated callus cells. When 10-d-old rice seedlings were treated with 0.025% methyl methanesulfonate (MMS) or 30 watts of UV-C light, they were apparently damaged by those genotoxic agents, with plants being more seriously injured by the latter. RNA gel blot analysis showed that the level of OsMRE11 mRNA remained unchanged during the 1- to 4-d incubation period with MMS. In contrast, OsMRE11 expression appeared to increase after 3 d of irradiation. In addition, treatments with salicylic acid and jasmonic acid, two important defense-related hormones, significantly activated the OsMRE11 gene. Based on these results, we discuss the possible functions of the OsMre11 protein in a mechanism by which the stability of rice chromosomes is maintained.

Original languageEnglish
Pages (from-to)229-236
Number of pages8
JournalJournal of Plant Biology
Volume48
Issue number2
DOIs
Publication statusPublished - 2005 Jun 30

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rice
methyl methanesulfonate
proteins
yeasts
chromosomes
DNA-binding domains
telomeres
jasmonic acid
tillers
salicylic acid
meristems
ultraviolet radiation
callus
genes
amino acid sequences
irradiation
hormones
gels
RNA
cells

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Hong, J. P., Sang, M. K., Moon, Y. R., Choe, S., Phun, B. P., An, G., & Kim, W. T. (2005). Structure and expression of OsMRE11 in rice. Journal of Plant Biology, 48(2), 229-236. https://doi.org/10.1007/BF03030412
Hong, Jong Pil ; Sang, Mi Kim ; Moon, Young Ryu ; Choe, Sunghwa ; Phun, Bum Park ; An, Gynheung ; Kim, Woo Taek. / Structure and expression of OsMRE11 in rice. In: Journal of Plant Biology. 2005 ; Vol. 48, No. 2. pp. 229-236.
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abstract = "In yeast and human cells, the Mre11 complex, which consists of Mre11, Rad50, and Xrs2/Nbs1 proteins, participates in basic aspects of chromosome metabolism, such as the repair of meiotic DNA breaks and telomere maintenance. In this study, we isolated a full-length cDNA clone, pOsMre11, encoding a rice ortholog of the Mre11 protein. Its predicted protein sequence (Mr = 79.2 kDa and pl value = 5.91) contains a metallo-phosphoesterase domain at its N-terminal region, and a single putative DNA binding domain in the central region of the protein, with significant homology to corresponding motifs in human and yeast Mre11 proteins. The OsMRE11 gene is constitutively expressed in all tissues examined here, including leaves, roots, tillers, and meristems, as well as in undifferentiated callus cells. When 10-d-old rice seedlings were treated with 0.025{\%} methyl methanesulfonate (MMS) or 30 watts of UV-C light, they were apparently damaged by those genotoxic agents, with plants being more seriously injured by the latter. RNA gel blot analysis showed that the level of OsMRE11 mRNA remained unchanged during the 1- to 4-d incubation period with MMS. In contrast, OsMRE11 expression appeared to increase after 3 d of irradiation. In addition, treatments with salicylic acid and jasmonic acid, two important defense-related hormones, significantly activated the OsMRE11 gene. Based on these results, we discuss the possible functions of the OsMre11 protein in a mechanism by which the stability of rice chromosomes is maintained.",
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Hong, JP, Sang, MK, Moon, YR, Choe, S, Phun, BP, An, G & Kim, WT 2005, 'Structure and expression of OsMRE11 in rice', Journal of Plant Biology, vol. 48, no. 2, pp. 229-236. https://doi.org/10.1007/BF03030412

Structure and expression of OsMRE11 in rice. / Hong, Jong Pil; Sang, Mi Kim; Moon, Young Ryu; Choe, Sunghwa; Phun, Bum Park; An, Gynheung; Kim, Woo Taek.

In: Journal of Plant Biology, Vol. 48, No. 2, 30.06.2005, p. 229-236.

Research output: Contribution to journalArticle

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T1 - Structure and expression of OsMRE11 in rice

AU - Hong, Jong Pil

AU - Sang, Mi Kim

AU - Moon, Young Ryu

AU - Choe, Sunghwa

AU - Phun, Bum Park

AU - An, Gynheung

AU - Kim, Woo Taek

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Hong JP, Sang MK, Moon YR, Choe S, Phun BP, An G et al. Structure and expression of OsMRE11 in rice. Journal of Plant Biology. 2005 Jun 30;48(2):229-236. https://doi.org/10.1007/BF03030412