Structure and expression of the rice glutelin multigene family.

T. W. Okita, Y. S. Hwang, J. Hnilo, Woo Taek Kim, A. P. Aryan, R. Larson, H. B. Krishnan

Research output: Contribution to journalArticle

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Abstract

A near full-length cDNA and three genomic clones for rice (Oryza sativa L.) glutelin were isolated and studied. Based on nucleic acid sequence and Southern blot analyses, the three isolated glutelin genomic clones were representative members of three gene subfamilies each containing five to eight copies. A comparison of DNA sequences displayed by relevant regions of these genomic clones showed that two subfamilies, represented by clones Gt1 and Gt2, were closely related and evolved by more recent gene duplication events. The 5'-flanking and coding sequences of Gt1 and Gt2 displayed at least 87% homology. In contrast, Gt3 showed little or no homology in the 5'-flanking sequences upstream of the putative CAAT boxes and exhibited significant divergence in all other portions of the gene. Conserved sequences in the 5'-flanking regions of these genes were identified and discussed in light of their potential regulatory role. The derived primary sequences of all three glutelin genomic clones showed significant homology to the legume 11 S storage proteins indicating a common gene origin. A comparison of the derived glutelin primary sequences showed that mutations were clustered in three peptide regions. One peptide region corresponded to the highly mutable hypervariable region of legume 11 S storage proteins, a potential target area for protein modification. Expression studies indicated that glutelin mRNA transcripts are differentially accumulated during endosperm development. Promoters of Gt2 and Gt3 were functional as they direct transient expression of chloramphenicol acetyltransferase in cultured plant cells.

Original languageEnglish
Pages (from-to)12573-12581
Number of pages9
JournalThe Journal of biological chemistry
Volume264
Issue number21
Publication statusPublished - 1989 Jan 1

Fingerprint

Glutens
Multigene Family
Clone Cells
Genes
5' Flanking Region
Protein S
Fabaceae
Nucleic acid sequences
Endosperm
Peptides
Proteins
Chloramphenicol O-Acetyltransferase
Gene Duplication
Conserved Sequence
DNA sequences
Plant Cells
Southern Blotting
Nucleic Acids
Cultured Cells
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Okita, T. W., Hwang, Y. S., Hnilo, J., Kim, W. T., Aryan, A. P., Larson, R., & Krishnan, H. B. (1989). Structure and expression of the rice glutelin multigene family. The Journal of biological chemistry, 264(21), 12573-12581.
Okita, T. W. ; Hwang, Y. S. ; Hnilo, J. ; Kim, Woo Taek ; Aryan, A. P. ; Larson, R. ; Krishnan, H. B. / Structure and expression of the rice glutelin multigene family. In: The Journal of biological chemistry. 1989 ; Vol. 264, No. 21. pp. 12573-12581.
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Okita, TW, Hwang, YS, Hnilo, J, Kim, WT, Aryan, AP, Larson, R & Krishnan, HB 1989, 'Structure and expression of the rice glutelin multigene family.', The Journal of biological chemistry, vol. 264, no. 21, pp. 12573-12581.

Structure and expression of the rice glutelin multigene family. / Okita, T. W.; Hwang, Y. S.; Hnilo, J.; Kim, Woo Taek; Aryan, A. P.; Larson, R.; Krishnan, H. B.

In: The Journal of biological chemistry, Vol. 264, No. 21, 01.01.1989, p. 12573-12581.

Research output: Contribution to journalArticle

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Okita TW, Hwang YS, Hnilo J, Kim WT, Aryan AP, Larson R et al. Structure and expression of the rice glutelin multigene family. The Journal of biological chemistry. 1989 Jan 1;264(21):12573-12581.