Structure and stress-related expression of two cDNAs encoding proteinase inhibitor II of Nicotiana glutinosa L.

Doil Choi, Jong A. Park, Young Sam Seo, Yoon Joo Chun, Woo Taek Kim

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Two cDNAs, pNGPI-1 and pNGPI-2, encoding Nicotiana glutinosa proteinase inhibitor II (PI-II) have been cloned, sequenced and identified. The deduced amino acid sequences are 54-82% identical to those of other plant PI-II. The NGPI-1 protein is composed of eight repeated domains, while NGPI-2 contains six repeated regions, each with a putative reactive site. The expression of NGPI-1 is highly regulated in a developmental- and tissue-specific manner, with the transcript being detected in young leaves and floral organs of N. glutinosa plants. In mature leaves, the NGPI-1 gene is rapidly activated by distinct temporal induction patterns in response to pathogen-related (biotic) and wound-related (abiotic) stresses. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)211-215
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1492
Issue number1
DOIs
Publication statusPublished - 2000 Jun 21

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Pathogens
Tobacco
Amino Acid Sequence
Catalytic Domain
Peptide Hydrolases
Complementary DNA
Genes
Tissue
Amino Acids
Wounds and Injuries
Proteins
plant proteinase inhibitor II protein

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

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title = "Structure and stress-related expression of two cDNAs encoding proteinase inhibitor II of Nicotiana glutinosa L.",
abstract = "Two cDNAs, pNGPI-1 and pNGPI-2, encoding Nicotiana glutinosa proteinase inhibitor II (PI-II) have been cloned, sequenced and identified. The deduced amino acid sequences are 54-82{\%} identical to those of other plant PI-II. The NGPI-1 protein is composed of eight repeated domains, while NGPI-2 contains six repeated regions, each with a putative reactive site. The expression of NGPI-1 is highly regulated in a developmental- and tissue-specific manner, with the transcript being detected in young leaves and floral organs of N. glutinosa plants. In mature leaves, the NGPI-1 gene is rapidly activated by distinct temporal induction patterns in response to pathogen-related (biotic) and wound-related (abiotic) stresses. Copyright (C) 2000 Elsevier Science B.V.",
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Structure and stress-related expression of two cDNAs encoding proteinase inhibitor II of Nicotiana glutinosa L. / Choi, Doil; Park, Jong A.; Seo, Young Sam; Chun, Yoon Joo; Kim, Woo Taek.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1492, No. 1, 21.06.2000, p. 211-215.

Research output: Contribution to journalArticle

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AB - Two cDNAs, pNGPI-1 and pNGPI-2, encoding Nicotiana glutinosa proteinase inhibitor II (PI-II) have been cloned, sequenced and identified. The deduced amino acid sequences are 54-82% identical to those of other plant PI-II. The NGPI-1 protein is composed of eight repeated domains, while NGPI-2 contains six repeated regions, each with a putative reactive site. The expression of NGPI-1 is highly regulated in a developmental- and tissue-specific manner, with the transcript being detected in young leaves and floral organs of N. glutinosa plants. In mature leaves, the NGPI-1 gene is rapidly activated by distinct temporal induction patterns in response to pathogen-related (biotic) and wound-related (abiotic) stresses. Copyright (C) 2000 Elsevier Science B.V.

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