Two cDNAs, pNGPI-1 and pNGPI-2, encoding Nicotiana glutinosa proteinase inhibitor II (PI-II) have been cloned, sequenced and identified. The deduced amino acid sequences are 54-82% identical to those of other plant PI-II. The NGPI-1 protein is composed of eight repeated domains, while NGPI-2 contains six repeated regions, each with a putative reactive site. The expression of NGPI-1 is highly regulated in a developmental- and tissue-specific manner, with the transcript being detected in young leaves and floral organs of N. glutinosa plants. In mature leaves, the NGPI-1 gene is rapidly activated by distinct temporal induction patterns in response to pathogen-related (biotic) and wound-related (abiotic) stresses. Copyright (C) 2000 Elsevier Science B.V.
|Number of pages||5|
|Journal||Biochimica et Biophysica Acta - Gene Structure and Expression|
|Publication status||Published - 2000 Jun 21|
Bibliographical noteFunding Information:
This work was supported by grants from the KOSEF HRC Project No. 1998G0203 to W.T.K. and the KOSEF through PMGBRC to D.C.
All Science Journal Classification (ASJC) codes
- Structural Biology