Studies of the relationship of the 230-kD and 180-kD bullous pemphigoid antigens

Mary Ann Robledo, SooChan Kim, Neil J. Korman, John R. Stanley, Ramzy S. Labib, Shozo Futamura, Grant J. Anhalt

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Abstract

In bullous pemphigoid (BP), autoantibodies from most patients recognize a high molecular weight 230-kD epidermal antigen (Ag) by immunoprecipitation. By Western immunoblotting, 50-70% of sera recognize the high molecular weight Ag, but 30-50% recognize a low molecular weight, 180-kD epidermal Ag. We examined the specificities of affinity-purified antibodies against these Ag. Antibodies specific for the 230-and 180-kD Ag were prepared by immunoaffinity against Ag immobilized on nitrocellulose and released by acid glycine. IgG eluted from the 230-kD Ag band retained its specific binding to the 230-kD Ag by immunoblotting, and bound to the epidermal basement membrane zone (BMZ) by indirect immunofluorescence (IF) and to hemidesmosomes by indirect immunoelectron microscopy (EM). IgG affinity purified by the 180-kD Ag band bound only the 180-kD Ag in immunoblotting, with no cross reaction to the 230-kD Ag, bound the epidermal BMZ by indirect IF, and also bound to hemidesmosomes in immuno-EM. IgG specific for the 230-kD Ag in immunoblotting immunoprecipitated only the 230-kD Ag, with no apparent precipitation of the 180-kD Ag. Surprisingly, IgG specific for the 180-kD Ag precipitated both the 180-and the 230-kD Ag in immunoprecipitation, and the 230-kD Ag band was much more intense than the 180-kD Ag band. This study shows that apparent cross-reactivity between these Ag by BP autoantibodies can only be detected in native conditions by immunoprecipitation, and cannot be demonstrated using denatured Ag in immunoblotting. The two proteins appear to be distinct Ag, closely associated in the epidermal hemidesmosome, but the exact relationship of these Ag to each other may not be clarified until complete structural data become available.

Original languageEnglish
Pages (from-to)793-797
Number of pages5
JournalJournal of Investigative Dermatology
Volume94
Issue number6
DOIs
Publication statusPublished - 1990 Jan 1

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Bullous Pemphigoid
Antigens
Hemidesmosomes
Immunoblotting
Immunoglobulin G
Immunoprecipitation
Molecular Weight
Molecular weight
Indirect Fluorescent Antibody Technique
Basement Membrane
Autoantibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Robledo, M. A., Kim, S., Korman, N. J., Stanley, J. R., Labib, R. S., Futamura, S., & Anhalt, G. J. (1990). Studies of the relationship of the 230-kD and 180-kD bullous pemphigoid antigens. Journal of Investigative Dermatology, 94(6), 793-797. https://doi.org/10.1111/1523-1747.ep12874652
Robledo, Mary Ann ; Kim, SooChan ; Korman, Neil J. ; Stanley, John R. ; Labib, Ramzy S. ; Futamura, Shozo ; Anhalt, Grant J. / Studies of the relationship of the 230-kD and 180-kD bullous pemphigoid antigens. In: Journal of Investigative Dermatology. 1990 ; Vol. 94, No. 6. pp. 793-797.
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abstract = "In bullous pemphigoid (BP), autoantibodies from most patients recognize a high molecular weight 230-kD epidermal antigen (Ag) by immunoprecipitation. By Western immunoblotting, 50-70{\%} of sera recognize the high molecular weight Ag, but 30-50{\%} recognize a low molecular weight, 180-kD epidermal Ag. We examined the specificities of affinity-purified antibodies against these Ag. Antibodies specific for the 230-and 180-kD Ag were prepared by immunoaffinity against Ag immobilized on nitrocellulose and released by acid glycine. IgG eluted from the 230-kD Ag band retained its specific binding to the 230-kD Ag by immunoblotting, and bound to the epidermal basement membrane zone (BMZ) by indirect immunofluorescence (IF) and to hemidesmosomes by indirect immunoelectron microscopy (EM). IgG affinity purified by the 180-kD Ag band bound only the 180-kD Ag in immunoblotting, with no cross reaction to the 230-kD Ag, bound the epidermal BMZ by indirect IF, and also bound to hemidesmosomes in immuno-EM. IgG specific for the 230-kD Ag in immunoblotting immunoprecipitated only the 230-kD Ag, with no apparent precipitation of the 180-kD Ag. Surprisingly, IgG specific for the 180-kD Ag precipitated both the 180-and the 230-kD Ag in immunoprecipitation, and the 230-kD Ag band was much more intense than the 180-kD Ag band. This study shows that apparent cross-reactivity between these Ag by BP autoantibodies can only be detected in native conditions by immunoprecipitation, and cannot be demonstrated using denatured Ag in immunoblotting. The two proteins appear to be distinct Ag, closely associated in the epidermal hemidesmosome, but the exact relationship of these Ag to each other may not be clarified until complete structural data become available.",
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Robledo, MA, Kim, S, Korman, NJ, Stanley, JR, Labib, RS, Futamura, S & Anhalt, GJ 1990, 'Studies of the relationship of the 230-kD and 180-kD bullous pemphigoid antigens', Journal of Investigative Dermatology, vol. 94, no. 6, pp. 793-797. https://doi.org/10.1111/1523-1747.ep12874652

Studies of the relationship of the 230-kD and 180-kD bullous pemphigoid antigens. / Robledo, Mary Ann; Kim, SooChan; Korman, Neil J.; Stanley, John R.; Labib, Ramzy S.; Futamura, Shozo; Anhalt, Grant J.

In: Journal of Investigative Dermatology, Vol. 94, No. 6, 01.01.1990, p. 793-797.

Research output: Contribution to journalArticle

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AU - Anhalt, Grant J.

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